Bone marrow-derived mesenchymal stem cells expressing the bFGF transgene promote axon regeneration and functional recovery after spinal cord injury in rats
Bone marrow-derived mesenchymal stem cells expressing the bFGF transgene promote axon regeneration and functional recovery after spinal cord injury in rats
复制标题
DOI：
10.1179/1743132810y.0000000031
复制
发表时间：
2011-09-01
影响因子：
1.9
通讯作者：
Huang, Zhu-Song
中科院分区：
文献类型：
--
作者：
Liu, Wen-Ge;Wang, Zhen-Yu;Huang, Zhu-Song
目的：目的：观察转染碱性成纤维细胞生长因子（bFGF）基因的骨髓间充质干细胞（BMSCs）移植对脊髓损伤大鼠神经功能的影响。方法：将96只SD大鼠随机分为4组：（1）pcDNA3.1-bFGF组;（2）pcDNA3.1组;（3）BMSCs组;（4）DMEM对照组。建立大鼠急性脊髓损伤（SCI）模型后，将1 × 10（6）BMSCs或转染pcDNA3.1-bFGF或pcDNA3.1的细胞注射到1 ~ 3组大鼠体内。在注射后第1、7、14和21天，使用Basso-Beattie-Bresnahan（BBB）运动评定量表评价运动功能的恢复。结果：DMEM组BBB评分明显低于1-3组（P
Objective: To investigate neurological effects of transplanting bone marrow-derived mesenchymal stem cells (BMSCs) transfected with the basic fibroblast growth factor (bFGF) gene in spinal cord-injured rats.Methods: Ninety-six male adult Sprague-Dawley rats were randomized into four groups: (1) pcDNA3.1-bFGF group; (2) pcDNA3.1 group; (3) BMSCs group; and (4) vehicle control (DMEM) group. After the rat model of acute spinal cord injury (SCI) was established, 1 x 10(6) BMSCs or cells transfected with pcDNA3.1-bFGF or pcDNA3.1 were injected into rats of groups 1-3. At days 1, 7, 14, and 21 after injection, the Basso-Beattie-Bresnahan (BBB) locomotor rating scale was used to evaluate recovery of motor function. Expression changes of bFGF, myelin basic protein (MBP), and NF200 were examined by immunohistochemistry.Results: The BBB score of DMEM group was significantly lower than those of groups 1-3 (P