Characterization of pemphigus foliaceus antigen from human epidermis.
Characterization of pemphigus foliaceus antigen from human epidermis.
复制标题
人表皮落叶型天疱疮抗原的表征。
DOI：
10.1111/1523-1747.ep12474454
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Diaz,LA
中科院分区：
文献类型：
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作者：
Calvanico,NJ;Martins,CR;Diaz,LA
天疱疮（PF）及其地方性形式，Fogo Selvagem (FS)，其特征是角膜下小泡和针对角化细胞表面抗原的致病性IgG自身抗体。主要的FS抗原仍然与不溶性表皮包膜部分结合。在本文中，我们证明了这种抗原可以通过超声从包膜部分释放出来。通过免疫沉淀可以检测到分子量（MW）分别为260、80、62和45 kD的四种组分。260-kD的成分通过煮沸或用甘氨酸HG提取而损失！pH值为2.8。其主要成分似乎是80- kd和62-kD多肽。它们作为一个单元在0.1% SDS中凝胶过滤，在115-120 kD的MW范围内进行色谱。FS抗原呈阳离子，在低pH下与SDS形成不溶性配合物，对硫酸铵和冻融不稳定。它不受正压浓度、50%丙酮沉淀和还原/烷基化的影响。FS抗原在所有FS和非地方性PE血清中沉淀，除了临床和血清学完全缓解的患者。在50%的寻常型天疱疮患者中也有FS抗原沉淀，但在大疱性类天疱疮血清中没有。所有FS抗原成分都被IgG4自身抗体免疫沉淀，但来自同一患者的IgG1亚类似乎只免疫沉淀62-kD多肽。ES抗原能够吸附人抗人粘粒蛋白1 （DG1）的自身抗体，但不能吸附兔抗牛DG1或dg2的抗血清。本文表明，物理应激，即超声，可能能够从表皮包膜组分中溶解足够的ES抗原，以进一步进行化学表征。这些PS抗原与其他已报道的FS抗原的关系目前尚不清楚。
Pemphigus foliaceus (PF) and its endemic form, Fogo Selvagem (FS), are characterized by subcorneal vesicles and pathogenic IgG autoantibodies directed against keratinocyte surface antigens. A major pool of FS antigen(s) remains bound to the insoluble epidermal envelope fraction. In this paper we demonstrate that this antigen(s) can be released From the envelope fraction by sonication. By immune precipitation four components can be detected, having molecular weights (MW) of 260, 80, 62, and 45 kD. The 260-kD component is lost by boiling or extraction with glycine HG! at pH 2.8. The major components appear to be the 80- and 62-kD poly-peptides. They chromatograph as a unit by gel filtration in 0.1% SDS, in the MW range of 115–120 kD. The FS antigen(s) appears to be cationic, forming insoluble complexes at low pH with SDS, and is labile to ammonium sulfate and freezing and thawing. It is unaffected by positive pressure concentration, 50% acetone precipitation, and reduction/alkylation. The FS antigen(s) is precipitated by all FS and nonendemic PE sera except those in complete clinical and serologic remission. The FS antigen(s) is also precipitated by 50% of pemphigus vulgaris but none of the bullous pemphigoid sera tested. All FS antigenic components are immunoprecipitated by IgG4 autoantibodies, but the IgG1 subclass from the same patients appear to immunoprecipitate only the 62-kD polypeptide. The ES antigen(s) is able to adsorb human autoantibodies against human desmoglein 1 (DG1), but not rabbit antisera against bovine DG1 or 2. This paper shows that physical stress, i.e., sonication, may be able to solubilize sufficient ES antigen(s) from the epidermal envelope fractions for further chemical characterization. The relationship of these PS antigen(s) to other reported FS antigens is presently unknown.