Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247.
Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247.
复制标题
DOI：
10.1007/s11274-017-2239-0
复制
发表时间：
2017-04
影响因子：
4.1
通讯作者：
Kishimoto N
中科院分区：
文献类型：
--
作者：
Kurata A;Yamaura Y;Tanaka T;Kato C;Nakasone K;Kishimoto N
从相模湾1171 m深的Calyptogena群落内的深海沉积物中新分离到一株Aneurinibacillus：sp. YR 247。该菌株对丝状真菌巴西曲霉（Aspergillus brasiliensis）NBRC 9455具有抗真菌活性。YR 247细胞经乙醇提取后得到的粗提物具有广泛的抗菌活性。抗真菌化合物在4-70 °C和pH 2.0-12.0下稳定。经蛋白酶K处理后，未检测到抑菌活性，表明菌株YR 247的抑菌化合物为肽类化合物。纯化的抗真菌化合物的电喷雾电离质谱表明，肽化合物的平均分子量为1167.9。菌株YR 247的抗真菌化合物的分子量与相关的动脉瘤芽孢杆菌和芽孢杆菌属细菌产生的抗菌肽的分子量不同。来自深海细菌Aneurinibacillus sp.YR247的抗真菌肽化合物可用作生物防治剂。
Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by ethanol extraction exhibited broad antimicrobial activities. The antifungal compound is stable at 4–70 °C and pH 2.0–12.0. After treatment with proteinase K, the antifungal activity was not detected, indicating that the antifungal compound of strain YR247 is a peptidic compound. Electrospray ionization mass spectrometry of the purified antifungal compound indicated that the peptidic compound has an average molecular weight of 1167.9. The molecular weight of the antifungal compound from strain YR247 is different from those of antimicrobial peptides produced by the related Aneurinibacillus and Bacillus bacteria. The antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 may be useful as a biocontrol agent.