Genomic alterations in lung adenocarcinomas detected by multicolor fluorescence in situ hybridization and comparative genomic hybridization
Genomic alterations in lung adenocarcinomas detected by multicolor fluorescence in situ hybridization and comparative genomic hybridization
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DOI：
10.1016/j.cancergencyto.2007.11.012
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发表时间：
2008-03-01
影响因子：
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通讯作者：
Shu, Yong-Qian
中科院分区：
文献类型：
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作者：
Shen, Hua;Zhu, Yu;Shu, Yong-Qian
我们使用两种分子细胞遗传学技术，荧光原位杂交（M-FISH）和比较基因组杂交（CGH），分析三个已建立的肺腺癌细胞系（A549，H1650和SPC-A-1）和原发性肺腺癌样本，以确定常见的染色体畸变。M-FISH显示了许多复杂的染色体重排。5、6、11、12和17号染色体最常参与染色体间的转移。易位CGH显示1 q、2 p、3q、5 p、5 q、7 p、8 q、11 q、12 q、14 q、16 p、17 p、19 q、20 q、21 q和22 q上的区域通常被过度代表，而2 q、3 p、4p、5 q、7 q、8 p、9 p、13 q、14 q和17 p上的区域被代表不足。最常见的增益被发现在l 6p 13（在50%的样品），和l 6p 13扩增与相对较差的分化和晚期。M-FISH和CGH可以成为鉴定肺癌基因组改变以及诊断的有力工具。这些高表达的区域可能含有参与肺腺癌发病机制的潜在候选基因。(C)2008年爱思唯尔公司All rights reserved.
We used two molecular cytogenetic techniques, multicolor fluorescence in situ hybridization (M-FISH) and comparative genomic hybridization (CGH), to analyze three established lung adenocarcinoma cell lines (A549, H1650, and SPC-A-1) and primary lung adenocarcinoma samples, to identify common chromosomal aberrations. M-FISH revealed numerous complex chromosomal rearrangements. Chromosomes 5, 6, 11, 12, and 17 were most frequently involved in interchromosomal. translocations. CGH revealed regions on 1q, 2p, 3q, 5p, 5q, 7p, 8q, 11q, 12q, 14q, 16p, 17p, 19q, 20q, 21q, and 22q to be commonly overrepresented and regions on 2q, 3p, 4p, 5q, 7q, 8p, 9p, 13q, 14q, and 17p, to be underrepresented. The most common gains were found in l6p13 (in 50% of samples), and l6p13 amplification was associated with relatively poor differentiation and late stage. M-FISH and CGH can be a powerful tool in identification of genomic alterations in lung cancer, as well as in diagnosis. The overrepresented regions may harbor potential candidate genes involved in lung adenocarcinoma pathogenesis. (C) 2008 Elsevier Inc. All rights reserved.