Effects of 17β-Estradiol Metabolites on Cell Cycle Events in MCF-7 Cells
Effects of 17β-Estradiol Metabolites on Cell Cycle Events in MCF-7 Cells
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17β-雌二醇代谢物对 MCF-7 细胞细胞周期事件的影响
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
J. Seegers
中科院分区：
文献类型：
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作者：
M. Lottering;M. Haag;J. Seegers
在MCF-7细胞中，每天暴露于10 nM水平的17 β-雌二醇（E2）、2-羟基雌二醇（2-OHE 2）或2-甲氧基雌二醇（2-MeOE 2）6次后，观察到不同的细胞生长效应。2-OHE 2显著促进细胞生长（P 220，000），受影响最显著。相比之下，E2和2-OHE 2的影响很小。我们认为，增强2-MeOE 2诱导的蛋白磷酸化在S期可能会影响S期的事件，随后导致有丝分裂的抑制。在G2/M过渡期的蛋白质合成出乎意料地增强2-OHE 2，并没有增强E2。[35 S]甲硫氨酸掺入到蛋白质中的顺序为M（r）32，000 - 46，000、47，000 - 50，000、58，000 - 67，000和83，000 - 89，000（P < 0.05）。2-MeOE 2无影响。这项研究的结果表明，2-OHE 2可能是更有效的促分裂原，而2-MeOE 2作为细胞抑制素。
Different cell growth effects were observed in MCF-7 cells after six daily exposures to either 17 beta-estradiol (E2), 2-hydroxyestradiol (2-OHE2), or 2-methoxyestradiol (2-MeOE2) at 10 nM levels. 2-OHE2 enhanced cell growth significantly (P 220,000), were most prominently affected. In comparison, E2 and 2-OHE2 had little effect. We suggest that the enhanced 2-MeOE2-induced protein phosphorylation during S-phase may affect S-phase events, which subsequently causes inhibition of mitosis. Protein synthesis during G2/M transition was unexpectedly enhanced by 2-OHE2 and was not enhanced by E2. [35S]Methionine incorporation into proteins in the order of M(r) 32,000-46,000, 47,000-50,000, 58,000-67,000, and 83,000-89,000 was significantly (P < 0.05) increased. 2-MeOE2 had no effect. The results of this study indicate that 2-OHE2 may be the more potent mitogen, whereas 2-MeOE2 acts as a cytostatin.