ENHANCEMENT OF INTERLEUKIN-6 CYTOSTATIC EFFECT ON HUMAN BREAST-CARCINOMA CELLS BY SOLUBLE IL-6 RECEPTOR FROM URINE AND REVERSION BY MONOCLONAL-ANTIBODY
ENHANCEMENT OF INTERLEUKIN-6 CYTOSTATIC EFFECT ON HUMAN BREAST-CARCINOMA CELLS BY SOLUBLE IL-6 RECEPTOR FROM URINE AND REVERSION BY MONOCLONAL-ANTIBODY
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DOI：
10.1016/1043-4666(92)90029-q
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发表时间：
1992-01-01
期刊：
影响因子：
3.8
通讯作者：
REVEL, M
中科院分区：
文献类型：
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作者：
NOVICK, D;SHULMAN, LM;REVEL, M
白细胞介素6受体可溶性尿蛋白（IL-6-R-CRP）是一种纯化的结合IL-6的尿蛋白，被鉴定为80 kDa IL-6细胞受体的截短的50 kDa可溶性形式。添加IL-6-R-IL-6增强阈下浓度的人重组IL-6对小鼠浆细胞瘤T1165的生长刺激。由于这种作用可能是由于人IL-6对小鼠细胞受体的亲和力较低，因此我们测试了IL-6-R-IFN对人细胞的作用。我们发现，IL-6对乳腺癌细胞的生长抑制作用增强IL-6-R-β加入这些人细胞，虽然他们拥有丰富的IL-6受体。使用IL-6-R-CRP，可以实现IL-6的完全生长抑制，并且细胞对低水平的IL-6变得更加敏感。这些作用可通过阻断IL-6与细胞结合的抗IL-6-R-R β单克隆抗体来预防。天然存在的IL-6-R-R可能有助于增加IL-6的生长调节作用。
Interleukin 6 receptor soluble urinary protein (IL-6-R-SUP), a purified urinary protein binding IL-6 and identified as a truncated 50 kDa soluble form of the 80 kDa IL-6 cellular receptor, was tested for its biological activity. Addition of IL-6-R-SUP enhances the growth stimulation of mouse plasmacytoma T1165 by subliminal concentrations of human recombinant IL-6. Since this effect could be due to a lower affinity of human IL-6 for the mouse cell receptor, we tested the effect of IL-6-R-SUP on human cells. We show that the growth-inhibitory effect of IL-6 on breast carcinoma cells is enhanced by addition of IL-6-R-SUP to these human cells although they possess abundant IL-6 receptors. With IL-6-R-SUP, complete growth inhibition by IL-6 could be achieved and the cells became more sensitive to low levels of IL-6. These effects were prevented by a monoclonal antibody against IL-6-R-SUP which blocks IL-6 binding to cells. The naturally occurring IL-6-R-SUP may help to increase the growth-regulatory action of IL-6.