Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells.
Noninvasive Assessment of Cell Fate and Biology in Transplanted Mesenchymal Stem Cells.
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移植间充质干细胞的细胞命运和生物学的无创评估。
DOI：
10.1007/978-1-4939-6756-8_18
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Rodriguez-Porcel,Martin
中科院分区：
文献类型：
--
作者：
Franchi,Federico;Rodriguez-Porcel,Martin
近年来，分子影像学已成为细胞再生医学的必要条件。分子成像技术的发展，如报告基因技术，越来越多地使非侵入性评估的命运和生物学的细胞后，心血管应用。在这种情况下，生物发光成像是临床前研究的小动物模型中最常用的成像方式。在这里，我们提出了一个报告基因成像方法的详细协议，用于监测间充质干细胞移植在心肌缺血再灌注损伤的小鼠模型的活力和生物学。
Recently, molecular imaging has become aconditio sine qua nonfor cell-based regenerative medicine. Developments in molecular imaging techniques, such as reporter gene technology, have increasingly enabled the noninvasive assessment of the fate and biology of cells after cardiovascular applications. In this context, bioluminescence imaging is the most commonly used imaging modality in small animal models of preclinical studies. Here, we present a detailed protocol of a reporter gene imaging approach for monitoring the viability and biology of Mesenchymal Stem Cells transplanted in a mouse model of myocardial ischemia reperfusion injury.