Differential expression of a novel gene in response to coronatine, methyl jasmonate, and wounding in the coi1 mutant of Arabidopsis
Differential expression of a novel gene in response to coronatine, methyl jasmonate, and wounding in the coi1 mutant of Arabidopsis
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DOI：
10.1104/pp.116.3.1037
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发表时间：
1998-03-01
期刊：
影响因子：
7.4
通讯作者：
Arruda, P
中科院分区：
文献类型：
--
作者：
Benedetti, CE;Costa, CL;Arruda, P
冠碱是一种由植物致病菌产生的植物毒素。研究表明，冠状碱在植物中具有类似茉莉酸甲酯（MeJA）的作用。MeJA是一种植物信号分子，参与应激反应，如伤害和病原体攻击。在拟南芥中，MeJA对花粉粒发育至关重要。对冠状碱和MeJA不敏感的拟南芥线圈突变体产生不育的雄花，并对伤害表现出改变的反应。利用差异显示技术，鉴定了一种在野生型植物中被冠状碱快速诱导而在卷材中不被诱导的信息，并克隆了相应的cDNA。冠状碱诱导基因ATHCOR1（用于拟南芥冠状碱诱导）在幼苗、成熟叶片、花和叶柄中表达，但在根中未检测到。该基因在卷材植物中的表达显著降低，表明COI1影响了其表达。在野生型植物中，通过MeJA和损伤诱导ATHCOR1的速度很快。ATHCOR1序列与已知蛋白无强同源性。然而，预测的多肽含有一个存在于几种细菌、动物和植物水解酶中的保守氨基酸序列，并包括一个潜在的ATP/ gtp结合位点基序（P-loop）。
Coronatine is a phytotoxin produced by some plant-pathogenic bacteria. It has been shown that coronatine mimics the action of methyl jasmonate (MeJA) in plants. MeJA is a plant-signaling molecule involved in stress responses such as wounding and pathogen attack. In Arabidopsis thaliana, MeJA is essential for pollen grain development. The coil (for coronatine-insensitive) mutant of Arabidopsis, which is insensitive to coronatine and MeJA, produces sterile male flowers and shows an altered response to wounding. When the differential display technique was used, a message that was rapidly induced by coronatine in wild-type plants but not in coil was identified and the corresponding cDNA was cloned. The coronatine-induced gene ATHCOR1 (for A. thaliana coronatine-induced) is expressed in seedlings, mature leaves, flowers, and siliques but was not detected in roots. The expression of this gene was dramatically reduced in coil plants, indicating that COI1 affects its expression. ATHCOR1 was rapidly induced by MeJA and wounding in wild-type plants. The sequence of ATHCOR1 shows no strong homology to known proteins. However, the predicted polypeptide contains a conserved amino acid sequence present in several bacterial, animal, and plant hydrolases and includes a potential ATP/GTP-binding-site motif (P-loop).