Pro-apoptotic effects of 1′-acetoxychavicol acetate in human breast carcinoma cells
Pro-apoptotic effects of 1′-acetoxychavicol acetate in human breast carcinoma cells
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DOI：
10.1016/j.toxlet.2007.07.008
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发表时间：
2007-09-28
影响因子：
3.5
通讯作者：
Kleiner, Heather E.
中科院分区：
文献类型：
--
作者：
Campbell, Cheryl T.;Prince, Misty;Kleiner, Heather E.
热带生姜化合物1 '-乙酰氧基胡椒酚乙酸酯（ACA）在几种模型中具有癌症化学预防特性，但其对乳腺癌的作用尚未得到充分评价。在这项研究中，ACA对人乳腺癌衍生的MCF-7和MDA-MB-231细胞活力的影响进行了评估，使用台盼蓝拒染分析。ACA以时间和剂量依赖性方式显著降低细胞活力，有效浓度为10-50 μ M。通过光学显微镜、4，6-二脒基-2-苯基吲哚二盐酸盐染色和使用流式细胞术可视化的膜联蛋白V/Alexa Fluor 488染色对细胞进行形态学检查来确认细胞凋亡。ACA还增加了MDA-MB-231细胞中活化形式的caspase-3的蛋白表达。添加抗氧化剂N-乙酰半胱氨酸，抗坏血酸，或trolox防止ACA使用台盼蓝摄取作为标志物引起的生存能力的丧失。这些结果表明ACA可能通过诱导细胞凋亡而对乳腺癌细胞具有潜在的抗肿瘤作用。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
The tropical ginger compound, 1 '-acetoxychavicol acetate (ACA) possesses cancer chemopreventive properties in several models but its effects on breast cancer have not been fully evaluated. In this study, the effects of ACA on human breast carcinoma-derived MCF-7 and MDA-MB-231 cell viability were assessed using trypan blue exclusion analysis. ACA significantly decreased cell viability in a time- and dose-dependent manner, with effective concentrations 10-50 mu M. Apoptosis was confirmed by morphological examination of cells through light microscopy, 4,6-diamidino-2-phenylindole dihydrochloride staining, and annexin V/Alexa Fluor 488 staining visualized using flow cytometry. ACA also increased protein expression of the activated form of caspase-3 in MDA-MB-231 cells. Addition of antioxidants N-acetyleysteine, ascorbic acid, or trolox prevented the loss of viability caused by ACA using trypan blue uptake as a marker. These results suggest ACA may have potential anticancer effects against breast carcinoma cells by inducing apoptosis. (C) 2007 Elsevier Ireland Ltd. All rights reserved.