Analysis of a mouse monoclonal antibody that reacts with a specific region of the human proximal tubule and subsets renal cell carcinomas.
Analysis of a mouse monoclonal antibody that reacts with a specific region of the human proximal tubule and subsets renal cell carcinomas.
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发表时间：
1989-12
期刊：
影响因子：
11.2
通讯作者：
N H Bander;C. L. Finstad;Carlos Cordon-Cardo;R. Ramsawak;Vaughan Ed;Willet F. Whitmore;Herbert F. Oettgen;Myron R. Melamed;L. Old
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作者：
N H Bander;C. L. Finstad;Carlos Cordon-Cardo;R. Ramsawak;Vaughan Ed;Willet F. Whitmore;Herbert F. Oettgen;Myron R. Melamed;L. Old
鼠单克隆抗体（mAb）F31检测肾癌细胞提取物酸性脂质部分中发现的热稳定抗原（URO-8）。在176个人细胞系上测定mAb F31反应性的血清学分析。mAb F31与45例肾癌中的38例（正常肾上皮原代培养物中的细胞亚群）、13例结肠癌中的2例、15例肺癌中的2例和5例卵巢癌中的4例反应。检测的其他上皮、造血或神经外胚层细胞系均无反应性。新鲜冷冻组织切片的免疫荧光和免疫过氧化物酶分析显示mAb F31在肾脏、胃肠道、胆小管、支气管上皮和皮肤中具有反应性。在肾脏内，mAb F31免疫反应性仅限于近端小管的直线部分。由先前表征的mAb以及mAb F31组成的一组将近曲小管细胞的抗原表型定义为URO-2+/URO-3+/URO-4+/URO-10+/URO-8-/URO-5-;近直小管细胞的抗原表型定义为URO-2+/URO-3+/URO-4+/URO-10-/URO-8+/URO-5-; Henle氏降支细支细胞为URO-2-/URO-3+或-/URO-4+/URO-10-/URO-8-/URO-5+。虽然成人近端肾小管细胞显示URO-8和URO-10的相互表达，胎儿肾近端肾小管祖细胞共表达两种抗原（URO-10+/URO-8+）。用这些抗体对50例肾癌标本进行分型。14例为URO-10+/URO-8-，10例为URO-10-/URO-8+，25例同时表达两种抗原（URO-10+/URO-8+）。这些表型与这些特定亚群分别来自近曲小管、直肠部或近曲小管祖细胞的来源一致。只有一个标本未能表达URO-8或URO-10。
Murine monoclonal antibody (mAb) F31 detects a heat-stable antigen (URO-8) found in the acidic lipid fraction of renal cancer cell extracts. Serological analysis of mAb F31 reactivity was assayed on 176 human cell lines. mAb F31 reacted with 38 of 45 renal cancers, a subpopulation of cells in primary cultures of normal renal epithelia, and two of 13 colon, two of 15 lung, and four of five ovarian cancers. No other epithelial, hematopoietic, or neuroectodermal cell lines tested were reactive. Immunofluorescence and immunoperoxidase analyses of fresh frozen tissue sections revealed mAb F31 reactivity in kidney, gastrointestinal tract, biliary canaliculi, bronchial epithelium, and skin. Within the kidney, mAb F31 immunoreactivity was confined to the straight portion of the proximal tubule. A panel composed of previously characterized mAbs as well as mAb F31 defines the antigenic phenotype of proximal convoluted tubular cells as URO-2+/URO-3+/URO-4+/URO-10+/URO-8-/URO-5-; proximal straight tubular cells as URO-2+/URO-3+/URO-4+/URO-10-/URO-8+/URO-5-; and cells of the descending thin limb of Henle as URO-2-/URO-3+ or -/URO-4+/URO-10-/URO-8-/URO-5+. While adult proximal tubular cells demonstrated reciprocal expression of URO-8 and URO-10, fetal kidney proximal tubule progenitor cells coexpressed both antigens (URO-10+/URO-8+). Fifty renal cancer specimens were typed with these antibodies. Fourteen cases were URO-10+/URO-8-, ten cases were URO-10-/URO-8+, and 25 cases expressed both antigens (URO-10+/URO-8+). These phenotypes are consistent with derivation of these particular subsets from the proximal convoluted tubule, the pars recta, or a proximal tubule progenitor cell, respectively. Only one specimen failed to express either URO-8 or URO-10.