A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions
A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions
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DOI：
10.1016/j.febslet.2004.06.102
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发表时间：
2004-09-10
期刊：
影响因子：
3.5
通讯作者：
Sonoike, K
中科院分区：
文献类型：
--
作者：
Hihara, Y;Muramatsu, M;Sonoike, K
Pirin是最近在真核生物中发现的一种蛋白质，作为转录辅助因子或作为一种与细胞凋亡相关的蛋白质。虽然Pirin在细菌和人类之间高度保守，但还没有关于原核生物Pirin同源基因的报道。我们在这里展示了编码Pirin同源基因的Pira(Sll1773)和邻近的基因PirB(Ssl3389)在高盐度和一些其他胁迫条件下在蓝藻聚胞藻中上调。PCC 6803。PirAB基因的诱导与细胞死亡无关，PIRA的阻断也不影响基因表达谱。PirAB基因的表达受到由pirR(Slr1871)编码的LysR家族转录调控因子的负调控，该转录调控因子位于pirAB的上游。DNA芯片分析表明，PIRR除了抑制PIRB和PIRR本身的表达外，还抑制了紧密定位的ORF、slr1870和MutS(Sll1772)的表达。(C)2004年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.