Asymmetric distribution of histones during Drosophila male germline stem cell asymmetric divisions.
Asymmetric distribution of histones during Drosophila male germline stem cell asymmetric divisions.
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DOI：
10.1007/s10577-013-9356-x
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发表时间：
2013-05
影响因子：
2.6
通讯作者：
Chen, Xin
中科院分区：
文献类型：
--
作者：
Tran, Vuong;Feng, Lijuan;Chen, Xin
关键词：
人们早就知道表观遗传变化是可遗传的。然而，除了DNA甲基化之外，对表观遗传的分子机制知之甚少。许多类型的干细胞经历不对称细胞分裂以产生自我更新的干细胞和定向分化的子细胞。尽管如此，干细胞是否以及如何保留其表观遗传记忆仍然是有待阐明的问题。在果蝇雄性生殖系干细胞（GSC）的不对称分裂过程中，我们的研究发现，在GSC中存在的组蛋白3（H3）被选择性地分离，而在DNA复制过程中新合成的H3则富集在分化的子细胞中。我们提出了一个两步模型来解释这种不对称的组蛋白分布。首先，在有丝分裂之前，预先存在的组蛋白和新合成的组蛋白在两组姐妹染色单体上有差异地分布。接下来，在有丝分裂期间，主要由预先存在的组蛋白组成的姐妹染色单体的集合被分离成GSC，而富含新合成的组蛋白的另一组姐妹染色单体被分配给致力于分化的子细胞。在这篇综述中，我们应用目前的知识表观遗传和不对称细胞分裂，告知我们的讨论潜在的分子机制和细胞基础，这种不对称的组蛋白分布模式。我们还将讨论这种现象是否有助于维持干细胞的身份和重置其他子细胞的染色质结构进行分化。
It has long been known that epigenetic changes are inheritable. However, except for DNA methylation, little is known about the molecular mechanisms of epigenetic inheritance. Many types of stem cells undergo asymmetric cell division to generate a self-renewed stem cell and a daughter cell committed for differentiation. Still, whether and how stem cells retain their epigenetic memory remain questions to be elucidated. During the asymmetric division of Drosophila male germline stem cell (GSC), our recent studies revealed that the preexisting histone 3 (H3) are selectively segregated to the GSC, whereas newly synthesized H3 deposited during DNA replication are enriched in the differentiating daughter cell. We propose a two-step model to explain this asymmetric histone distribution. First, prior to mitosis, preexisting histones and newly synthesized histones are differentially distributed at two sets of sister chromatids. Next, during mitosis, the set of sister chromatids that mainly consist of preexisting histones are segregated to GSCs, while the other set of sister chromatids enriched with newly synthesized histones are partitioned to the daughter cell committed for differentiation. In this review, we apply current knowledge about epigenetic inheritance and asymmetric cell division to inform our discussion of potential molecular mechanisms and the cellular basis underlying this asymmetric histone distribution pattern. We will also discuss whether this phenomenon contributes to the maintenance of stem cell identity and resetting chromatin structure in the other daughter cell for differentiation.
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影响因子：
10.5
作者：
Drane, Pascal;Ouararhni, Khalid;Hamiche, Ali
通讯作者：
Hamiche, Ali
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.172403699
发表时间：
2002-12-10
影响因子：
11.1
作者：
Ahmad, K;Henikoff, S
通讯作者：
Henikoff, S
影响因子：
3.7
作者：
Fei, Ji-Feng;Huttner, Wieland B.
通讯作者：
Huttner, Wieland B.
DOI：
10.1126/science.1191078
发表时间：
2010-10-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bonasio R;Tu S;Reinberg D
通讯作者：
Reinberg D