Anaerobic/oxic/anoxic granular sludge process as an effective nutrient removal process utilizing denitrifying polyphosphate-accumulating organisms
Anaerobic/oxic/anoxic granular sludge process as an effective nutrient removal process utilizing denitrifying polyphosphate-accumulating organisms
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DOI：
10.1016/j.watres.2006.04.037
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发表时间：
2006-07-01
期刊：
影响因子：
12.8
通讯作者：
Sudo, Ryuichi
中科院分区：
文献类型：
--
作者：
Kishida, Naohiro;Kim, Juhyun;Sudo, Ryuichi
在生物脱氮除磷工艺中，利用聚磷菌（DNPAOs）具有有效利用有机碳基质和污泥产量低等优点。本研究提出了厌氧/好氧/缺氧颗粒污泥（AOAGS）工艺，作为一种适合于生物保护区利用DNPAOs的工艺。尽管对硝化细菌进行曝气，但AOAGS工艺可以产生适合于DNPAO培养的厌氧/缺氧条件，因为缺氧区存在于好氧相中的颗粒污泥内部。因此，DNPAO可以与硝化细菌在单个反应器中共存。此外，通过在好氧相之后添加缺氧相，可以改善反应器中DNPAO的可用性。这些特性使得AOAGS工艺能够实现有效的脱氮除磷。以乙酸盐为基础的合成废水（COD：600 mg/L，NH 4-N：60 mg/L，PO 4-P：10 mg/L）为进水，在厌氧/好氧/缺氧循环条件下，在1个月内成功形成了直径为500 μ m的颗粒污泥。虽然在好氧阶段结束时氮和磷的去除几乎完成，但在好氧阶段之后的短缺氧期对于氮和磷的进一步去除是必要的。结果表明，NH 4-N、NOx-N和PO 4-P的出水浓度始终低于1 mg/L。通过微传感器测量发现，氧在颗粒污泥中的渗透深度约为100 μ m。此外，从荧光原位杂交的微生物分析来看，聚磷菌的存在深度大于最大透氧深度。水质数据、氧分布和微生物群落结构表明，颗粒污泥内的DNPAO可能负责好氧阶段的反硝化，这使得AOAGS工艺能够有效去除营养物。(c)2006爱思唯尔有限公司保留所有权利。
In a biological nutrient removal (BNR) process, the utilization of denitrifying polyphosphate-accumulating organisms (DNPAOs) has many advantages such as effective use of organic carbon substrates and low sludge production. As a suitable process for the utilization of DNPAOs in BNR, an anaerobic/oxic/anoxic granular sludge (AOAGS) process was proposed in this study. in spite of performing aeration for nitrifying bacteria, the AOAGS process can create anaerobic/anoxic conditions suitable for the cultivation of DNPAOs because anoxic zones exist inside the granular sludge in the oxic phase. Thus, DNPAOs can coexist with nitrifying bacteria in a single reactor. In addition, the usability of DNPAOs in the reactor can be improved by adding the anoxic phase after the oxic phase. These characteristics enable the AOAGS process to attain effective removal of both nitrogen and phosphorus. When acetate-based synthetic wastewater (COD: 600 mg/L, NH4-N: 60 mg/L, PO4-P: 10mg/L) was supplied to a laboratory-scale sequencing batch reactor under the operation of anaerobic/oxic/anoxic cycles, granular sludge with a diameter of 500 Pin was successfully formed within 1 month. Although the removal of both nitrogen and phosphorus was almost complete at the end of the oxic phase, a short anoxic period subsequent to the oxic phase was necessary for further removal of nitrogen and phosphorus. As a result, effluent concentrations of NH4-N, NOx-N and PO4-P were always lower than 1 mg/L. It was found that penetration depth of oxygen inside the granular sludge was approximately 100 mu m by microsensor measurements. in addition, from the microbiological analysis by fluorescence in situ hybridization, existence depth of polyphosphate-accumulating organisms was further than the maximum oxygen penetration depth. The water quality data, oxygen profiles and microbial community structure demonstrated that DNPAOs inside the granular sludge may be responsible for denitrification in the oxic phase, which enables effective nutrient removal in the AOAGS process. (c) 2006 Elsevier Ltd. All rights reserved.