Gene expression profile analysis of AIDS-related primary effusion lymphoma (PEL) suggests a plasmablastic derivation and identifies PEL-specific transcripts

Gene expression profile analysis of AIDS-related primary effusion lymphoma (PEL) suggests a plasmablastic derivation and identifies PEL-specific transcripts
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DOI：
10.1182/blood-2002-10-3090
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发表时间：
2003-05-15
期刊：
BLOOD
影响因子：
20.3
通讯作者：
Carbone, A
Carbone, A
中科院分区：
医学1区
文献类型：
--
作者：
Klein, U;Gloghini, A;Carbone, A

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艾滋病相关的原发性渗出性淋巴瘤（PEL）是一种与艾滋病相关的恶性肿瘤，其特征是肿瘤细胞能够特异性地在浆液性体腔中安家。在这里，我们使用基因表达谱分析（约12 000个基因），以进一步确定表型的PEL和研究淋巴瘤的关系，正常B细胞和其他肿瘤亚型，包括非霍奇金淋巴瘤（NHL）的免疫功能正常的主机和艾滋病相关的NHL（艾滋病-NHL）。结果表明，PEL显示出一个共同的基因表达谱，这是明确不同的免疫活性宿主和艾滋病NHL亚型的所有NHL，而与那些，是不相关的生发中心（GC）或记忆B细胞。PEL的基因表达谱被定义为浆细胞，因为它显示了由EB病毒（EBV）转化的淋巴母细胞样细胞系和AIDS免疫母细胞淋巴瘤鉴定的免疫母细胞和由多发性骨髓瘤细胞系定义的浆细胞的特征。最后，我们的研究结果确定了一组在PEL肿瘤细胞中特异性表达的基因。在蛋白水平上验证了它们的表达，提示了它们潜在的致病和临床意义。
AIDS-related primary effusion lymphoma (PEL) is an HIV-associated malignancy characterized by the ability of the tumor cells to specifically home in the serous body cavities. Here we used gene expression profile analysis (about 12 000 genes) to further define the phenotype of PEL and to investigate the lymphoma relationship to normal B cells and to other tumor subtypes, including non-Hodgkin lymphomas (NHLs) of immunocompetent hosts and AIDS-associated NHL (AIDS-NHL). The results showed that PEL displayed a common gene expression profile that is clearly distinct from all NHLs of immunocompetent hosts and AIDS-NHL subtypes and, in contrast to those, is not related to germinal center (GC) or memory B cells. The gene expression profile of PEL was defined as plasmablastic because it showed features of both immunoblasts identified by Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines and AIDS immunoblastic lymphoma, and plasma cells, as defined by multiple myeloma cell lines. Finally, our results identify a set of genes specifically expressed in PEL tumor cells. Their expression was validated at the protein level, suggesting their potential pathogenetic and clinical significance.