Antibody mediated suppression of secondary IgM response in nude mice against vesicular stomatitis virus.
Antibody mediated suppression of secondary IgM response in nude mice against vesicular stomatitis virus.
复制标题
抗体介导抑制裸鼠体内针对水泡性口炎病毒的继发性 IgM 反应。
作者：
S. Charan;R. Zinkernagel
用水泡性口炎病毒（VSV）的两种血清型（印第安纳州VSV（VSV-Ind）或新泽西VSV（VSV-NJ））中的任一种免疫裸鼠，显示出与正常正常胸腺小鼠相当的早期T细胞非依赖性免疫球蛋白（IG）M抗体应答。然而，与正常胸腺小鼠不同，再次注射同源血清型的裸鼠无法产生第二次可测量的血清中和（SN）抗体应答;第二次注射异源血清型诱导了正常的一级SN抗体应答。在初次感染后约10周，对第二次挑战的特异性不应性恢复。当通过酶联免疫吸附试验（ELISA）测定抗体反应时，即使在用异源血清型进行挑战后，也可以观察到先前免疫的抑制作用;这一发现可能反映了已知存在两种血清型之间共享的共同非中和决定簇。在裸鼠初次应答期间观察到弱的2-巯基乙醇（2-ME）耐药抗VSV IgG SN抗体应答，并且在ELISA中也发现了这种应答;在二次感染后，未出现这种2-ME耐药应答。在裸鼠和+/+小鼠中的血清转移研究证实，裸鼠中第二次应答的表型特异性短暂不应性是通过抗VSV特异性IgM抗体介导的。
Nude mice immunized with either of the two serotypes of vesicular stomatitis virus (VSV), VSV Indiana (VSV-Ind) or VSV-New Jersey (VSV-NJ), showed an early T cell independent immunoglobulin (Ig) M antibody response comparable with normal euthymic mice. Unlike euthymic mice, however, nude mice reinjected with the homologous serotype were unable to mount a second measurable serum neutralizing (SN) antibody response; a second injection with the heterologous serotype induced a normal primary type of SN antibody response. The serotype-specific refractoriness to a second challenge recovered at about 10 wk after primary infection. When antibody responses were assayed by enzyme-linked immunoabsorbent assay (ELISA), suppressive effects by previous immunization could be observed even after challenge with the heterologous serotypes; this finding probably reflects the known existence of common nonneutralizing determinants shared between the two serotypes. A weak 2-mercaptoethanol (2-ME)-resistant anti-VSV IgG SN antibody response was noticed during the primary response in nude mice and was also found in ELISA; after second infections, this 2-ME-resistant response did not develop. Serum transfer studies in nude and +/+ mice confirmed that the serotype-specific transitory refractoriness of a second response in nude mice was mediated through the anti-VSV-specific IgM antibodies.