Preparation of type III procollagen and collagen from rat skin.
Preparation of type III procollagen and collagen from rat skin.
复制标题
从大鼠皮肤中制备 III 型原胶原和胶原蛋白。
DOI：
10.1021/bi00722a030
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
G. Martin
中科院分区：
文献类型：
--
作者：
P. Byers;K. McKenney;J. Lichtenstein;G. Martin
中性盐溶液优先从大鼠皮肤中提取I型和III型胶原的前体形式。这些蛋白质通过DEAE-纤维素层析分离，并根据溴化氰裂解产生的肽鉴定。分离出这些提取物中存在的一些III型胶原蛋白，发现其含有与用胃蛋白酶有限消化后从皮肤中分离的III型胶原蛋白中的那些在大小和组成上相似的链。在脊椎动物组织中发现的几种类型的胶原蛋白的前体链的两种形式，前体（III）和生物合成前体（前胶原）已被鉴定。其中包括皮肤、骨和腱的主要胶原蛋白的前体（I型前胶原）（Lay-man等，1971; Bellamy和Bornstein，1971）、软骨特异性胶原（II型前胶原）（Dehm和Prockop，1973）和基底膜的胶原组分（IV型前胶原）（Grant等，1972年）。米勒等人1971年，他提出了另一个假设。
Neutral salt solutions preferentially extract precursor forms of type I and III collagens from rat skin. These proteins were isolated by chromatography on DEAE-cellulose and identified on the basis of the peptides pro-duced by cleavage with cyanogen bromide. Some type III collagen present in these extracts was isolated and found to contain a chains similar in size and composition to those in type III collagen isolated from skin after limited digestion with pepsin. Two forms of precursor chains, pro al (III) and-Biosynthetic precursors (procollagens) of several types of collagen found in vertebrate tissues have been identified. Among these are the precursors of the major collagenous protein of skin, bone, and tendon (type I procollagen)(Lay-man et al. 1971; Bellamy and Bornstein 1971), the carti-lage specific collagen (type II procollagen)(Dehm and Prockop 1973), and a collagenous component of basement membranes (type IV procollagen)(Grant et al., 1972). Miller et al.(1971) postulated the existence of another