Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system.
Production of yeast (m2G10) methyltransferase (Trm11 and Trm112 complex) in a wheat germ cell-free translation system.
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DOI：
10.1093/nass/nrp152
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Hori, Hiroyuki
中科院分区：
文献类型：
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作者：
Okada, Kazuki;Muneyoshi, Yuki;Hori, Hiroyuki
转移 RNA (鸟嘌呤-N(2)-)-甲基转移酶 [tRNA (m(2)G10) 甲基转移酶] 催化 tRNA 中 10 位 (G10) 鸟嘌呤 N(2)-原子的甲基转移，并在 10 位 (m(2)G10) 生成 N(2)-甲基鸟嘌呤。酵母酶含有两个蛋白质亚基（Trm11 和 Trm112）。 Trm11 蛋白预计是一个催化亚基，Trm112 包含一个锌指。在酵母细胞中，Trm112 不仅与 Trm11 结合，还与其他蛋白质（如 Lys9、Trm9 和 Mtq2）结合。因此，Trm112 蛋白可以调节多种蛋白复合物的数量。为了解决这些问题，我们开始了Trm112相关蛋白复合物合成的研究。在本次会议中，我们报告了在小麦胚芽无细胞翻译系统中合成活性 Trm11-Trm112 复合物。
Transfer RNA (guanine-N(2)-)-methyltransferase [tRNA (m(2)G10) methyltransferase] catalyzes a methyl-transfer from S-adenosyl-L-methionine to N(2)-atom of guanine at position 10 (G10) in tRNA and generates N(2)-methylguanine at position 10 (m(2)G10). Yeast enzyme contains two protein subunits (Trm11 and Trm112). Trm11 protein is expected to be a catalytic subunit and Trm112 contains a Zinc-finger. In yeast cells, Trm112 binds not only to Trm11 but also to other proteins such as Lys9, Trm9, and Mtq2. Therefore, the Trm112 protein may regulate population of several protein complexes. To address these issues, we started the study on synthesis of Trm112 related protein complexes. In this meeting, we report synthesis of active Trm11-Trm112 complex in a wheat germ cell-free translation system.