Noninvasive in vivo imaging of pancreatic islet cell biology
Noninvasive in vivo imaging of pancreatic islet cell biology
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DOI：
10.1038/nm1701
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发表时间：
2008-05-01
期刊：
影响因子：
82.9
通讯作者：
Berggren, Per-Olof
中科院分区：
文献类型：
--
作者：
Speier, Stephan;Nyqvist, Daniel;Berggren, Per-Olof
生物医学研究中细胞水平上复杂生物过程的研究。在这里，我们介绍一种利用眼睛前房作为自然身体窗口对胰岛进行无创体内荧光成像的新技术平台。移植到小鼠眼睛虹膜上的胰岛变得血管化，保留细胞成分，对刺激做出反应并恢复糖尿病。激光扫描显微镜可以以细胞分辨率对胰岛血管化、β细胞功能和死亡进行重复体内成像。因此，我们的结果为复杂细胞过程（如β细胞刺激-反应耦合）的无创体内研究奠定了基础，这些过程可以在生理和病理条件下纵向进行。
the study of complex biological processes at the cellular level in biomedical research. Here, we introduce a new technical platform for noninvasive in vivo fluorescence imaging of pancreatic islets using the anterior chamber of the eye as a natural body window. Islets transplanted into the mouse eye engrafted on the iris, became vascularized, retained cellular composition, responded to stimulation and reverted diabetes. Laser-scanning microscopy allowed repetitive in vivo imaging of islet vascularization, beta cell function and death at cellular resolution. Our results thus establish the basis for noninvasive in vivo investigations of complex cellular processes, like beta cell stimulus-response coupling, which can be performed longitudinally under both physiological and pathological conditions.