The characteristics of dorsal-root ganglia and sensory innervation of the hip in rats
The characteristics of dorsal-root ganglia and sensory innervation of the hip in rats
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DOI：
10.1302/0301-620x.90b2.19808
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发表时间：
2008-02-01
影响因子：
--
通讯作者：
Harada, Y.
中科院分区：
文献类型：
--
作者：
Nakajima, T.;Ohtori, S.;Harada, Y.
采用逆行神经传递和免疫组织化学方法研究了大鼠髋关节背根神经节感觉神经元的特征。将氟金溶液（FG）注射于10只大鼠左臀部。7天后，从T12和L6之间的两侧收获DRG。计数fg标记的降钙素基因相关肽免疫反应或分离素b4结合神经元的数量。fg标记的神经元分布在T13和L5之间的左侧DRGs中，主要分布在L2、L3和L4。在T13和L5之间的任何一侧DRGs中都存在少量fg标记的分离素b4结合神经元，但降钙素基因相关肽免疫反应神经元占所有fg标记神经元的30%。我们的研究结果可以解释从臀部到大腿或小腿相应的L2， L3和L4水平的疼痛。由于大多数神经元是与降钙素基因相关的含有肽免疫反应性肽的神经元，它们可能作为肽能DRG神经元在髋关节疼痛感知中发挥更重要的作用。
Using a rat model the characteristics of the sensory neurones of the dorsal-root ganglia (DRG) innervating the hip were investigated by retrograde neurotransport and immunohistochemistry.Fluoro-Gold solution (FG) was injected into the left hip of ten rats. Seven days later the DRG from both sides between T12 and L6 were harvested. The number of FG-labelled calcitonin gene-related peptide-immunoreactive or isolectin B4-binding neurones were counted.The FG-labelled neurones were distributed throughout the left DRGs between T13 and L5, primarily at L2, L3, and L4. Few FG-labelled isolectin B4-binding neurones were present in the DRGs of either side between T13 and L5, but calcitonin gene-related peptide-immunoreactive neurones made up 30% of all FG-labelled neurones.Our findings may explain the referral of pain from the hip to the thigh or lower leg corresponding to the L2, L3 and L4 levels. Since most neurones are calcitonin gene-related peptide-immunoreactive peptide-containing neurones, they may have a more significant role in the perception of pain in the hip as peptidergic DRG neurones.