In vitro characterization of natural and synthetic dermal matrices cultured with human dermal fibroblasts
In vitro characterization of natural and synthetic dermal matrices cultured with human dermal fibroblasts
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DOI：
10.1016/j.biomaterials.2003.09.058
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发表时间：
2004-06-01
期刊：
影响因子：
14
通讯作者：
Hutmacher, DW
中科院分区：
文献类型：
--
作者：
Ng, KW;Khor, HL;Hutmacher, DW
理想的真皮基质应该能够提供正确的生物和物理环境，以确保均匀的细胞和细胞外基质（ECM）分布，以及所需新组织的正确大小和形态。在体外评价了四种天然和合成3D基质作为真皮基质，即（1）马胶原泡沫，TissuFleece（R），（2）无细胞真皮替代物，Alloderm（R），（3）针织聚（乳酸-乙醇酸共聚物）（10：90）-聚（ε-己内酯）（PLGA-PCL）网片，（4）壳聚糖支架。人皮肤成纤维细胞培养3周以上的标本。通过电子显微镜、组织学和激光共聚焦显微镜评估细胞形态、分布和活力。通过MTS代谢测定和[H-3]-胸苷摄取分析代谢活性和DNA合成，同时通过免疫组织化学测定ECM蛋白表达。TissuFleece（R）. Alloderm（R）和PLGA-PCL网支持细胞附着、增殖和新组织形成。然而. TissuFleece（R）收缩至原始尺寸的10%，而Alloderm（R）主要支持材料表面的细胞增殖。PLGA-PCL补片促进了更均匀的细胞分布和组织形成。壳聚糖支架不支持细胞附着和增殖。这些结果表明，包括多孔性和机械稳定性的物理特性，以承受细胞收缩力是重要的，在决定成功的真皮基质材料。(C)2003爱思唯尔有限公司。保留所有权利。
The ideal dermal matrix should be able to provide the right biological and physical environment to ensure homogenous cell and extracellular matrix (ECM) distribution, as well as the right size and morphology of the neo-tissue required. Four natural and synthetic 3D matrices were evaluated in vitro as dermal matrices, namely (1) equine collagen foam, TissuFleece(R), (2) acellular dermal replacement, Alloderm(R), (3) knitted poly(lactic-co-glycolic acid) (10:90)-poly(epsilon-caprolactone) (PLGA-PCL) mesh, (4) chitosan scaffold. Human dermal fibroblasts were cultured on the specimens over 3 weeks. Cell morphology, distribution and viability were assessed by electron microscopy, histology and confocal laser microscopy. Metabolic activity and DNA synthesis were analysed via MTS metabolic assay and [H-3]-thymidine uptake, while ECM protein expression was determined by immunohistochemistry. TissuFleece(R). Alloderm(R) and PLGA-PCL mesh supported cell attachment, proliferation and neo-tissue formation. However. TissuFleece(R) contracted to 10% of the original size while Alloderm(R) supported cell proliferation predominantly on the surface of the material. PLGA-PCL mesh promoted more homogenous cell distribution and tissue formation. Chitosan scaffolds did not support cell attachment and proliferation. These results demonstrated that physical characteristics including porosity and mechanical stability to withstand cell contraction forces are important in determining the success of a dermal matrix material. (C) 2003 Elsevier Ltd. All rights reserved.