A fluorescent sensor for discrimination of HSA from BSA through selectivity evolution
A fluorescent sensor for discrimination of HSA from BSA through selectivity evolution
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一种通过选择性进化区分 HSA 和 BSA 的荧光传感器
DOI：
10.1016/j.aca.2018.09.010
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发表时间：
2018
影响因子：
6.2
通讯作者：
Zhu Hai-Liang
中科院分区：
文献类型：
--
作者：
Xu Yun-Jie;Su Mi-Mi;Li Hong-Lin;Liu Qi-Xing;Xu Chen;Yang Yu-Shun;Zhu Hai-Liang
许多疾病的临床前诊断需要定量检测人血清白蛋白（HSA）。本文从经典的“效应子-π-触发子”（Effector-π-Trigger）模式出发，经过两轮选择性进化，筛选出了一种高选择性的HSA传感器RhHSA，该传感器具有选择性高（HSA：BSA = 1：10时为1.6倍）、灵敏度高（检测限<5 nM，增强700倍以上）、稳定性好（24 h以上）和线性范围宽（0-0.5 mg/mL，适用于常规的HSA检测）等优点。检测系统不受介质极性或粘度的影响。HSA的破坏、位点竞争和分子对接为rhHSA能够嵌入布洛芬和保泰松位点提供了可靠的证据。这些提示也支持区分HSA和BSA。在活体细胞中的稳定补液和在尿液系统中的测定都预示着RhHSA在生物学上的应用潜力。
Pre-clinical diagnosis of many diseases required quantitative detection of Human Serum Albumin (HSA). Herein a high-selective HSA sensorRhHSAwas picked through a two-round selectivity evolution from the typical "Effector-π-Trigger" style.RhHSAsuggested advantages including high selective (∼6 fold for HSA:BSA = 1:10), sensitive (LOD ∼ 5 nM, over 700-fold enhancement), steady (over 24 h) and wide linear range (0–0.5 mg/mL, applicative for conventional HSA measurement). The detecting system was free from media polarity or viscosity. HSA destruction, site competition and molecular docking provided reliable evidence for the fact thatRhHSAcould be embedded into both ibuprofen and phenylbutazone sites of HSA. These hints also supported the discrimination of HSA from BSA. Stepwisely fluid replacement in living cells and measuring in urine system both inferred the potential ofRhHSAin biological applications.