Bio-initiated light addressable potentiometric sensor for unlabeled biodetection and its MEDICI simulation.
Bio-initiated light addressable potentiometric sensor for unlabeled biodetection and its MEDICI simulation.
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DOI：
10.1039/c1an15133a
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发表时间：
2011-10
期刊：
影响因子：
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通讯作者：
Yunfang Jia;C. Gao;Daofu Feng;Ming Wu;Yang Liu;Xinjuan Chen;Ke-li Xing;Xizeng Feng
中科院分区：
文献类型：
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作者：
Yunfang Jia;C. Gao;Daofu Feng;Ming Wu;Yang Liu;Xinjuan Chen;Ke-li Xing;Xizeng Feng
采用L-3，4-二羟基苯丙氨酸（L-DOPA）仿生锚定方法对结构为Si（3）N（4）/SiO（2）/Si的光寻址电位传感器（LAPS）表面进行活化。进行X射线光电子能谱（XPS）测量以确定其存在。用我们的LAPS系统测试了蛋白质在L-DOPA引发的LAPS上的固定化。然后将L-DOPA激活的LAPS用于未标记兔抗小鼠免疫球蛋白（IgG）的检测。L-DOPA激活的LAPS对抗原（Ag）的最大灵敏度约为5.68 nA/p[Ag]。当浓度在0-4 μg/mL（-1）范围内变化时，IgG测量中的LAPS响应为95 - 180 nA。这些实验表明L-DOPA是一种有效的LAPS表面改性材料。同时，进行基于MEDICI（Synopsys™）的模拟。模拟曲线与实验数据吻合较好，验证了我们对实验现象的理论分析，表明了MEDICI模拟生物电子器件的可行性。
A bio-mimetic anchoring strategy based on L-3,4-dihydroxyphenylalanine (L-DOPA) was exploited to activate the surface of light addressable potentiometric sensor (LAPS), with the structure of Si(3)N(4)/SiO(2)/Si. X-Ray photoelectron spectroscopy (XPS) measurements were carried out to ascertain its existence. The protein's immobilization on L-DOPA-initiated LAPS were also tested by our LAPS system. Then L-DOPA-activated LAPS were applied in the unlabeled rabbit anti-mouse immunoglobulin (IgG) detection. The maximum sensitivity of L-DOPA-activated LAPS to antigen (Ag) is about 5.68 nA/p[Ag]. LAPS responses in IgG measurements were from 95 to 180 nA, when the concentration was varied from 0-4 μg mL(-1). These experiments show that L-DOPA is an available material for LAPS surface modifications. At the same time, simulations based on MEDICI (Synopsys™) were performed. The simulated curves are in accordance with experimental data which demonstrate our theoretical analysis for the experimental phenomenon, and indicate the feasibility of simulating biological electronic devices with MEDICI.