Functional screening of intracardiac cell transplants using two-photon fluorescence microscopy.
Functional screening of intracardiac cell transplants using two-photon fluorescence microscopy.
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DOI：
10.1007/s00246-012-0314-8
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发表时间：
2012-08
影响因子：
1.6
通讯作者：
Rubart, Michael
中科院分区：
文献类型：
--
作者：
Tao, Wen;Soonpaa, Mark H.;Field, Loren J.;Chen, Peng-Sheng;Firulli, Anthony B.;Shou, Weinian;Rubart, Michael
关键词：
尽管成年哺乳动物心肌表现出有限的再生生长能力，但其内在更新率不足以补偿心脏病期间心肌细胞的损失。供体心肌细胞或心肌干细胞的移植被认为是重建心脏质量的一种有前途的策略，只要移植的细胞与宿主心肌功能性整合并积极促进其收缩力。我们之前开发了一种基于双光子荧光显微镜的测定法，可以在心内递送后对供体细胞功能进行原位筛选。在这里，我们回顾了这些技术并总结了其在定量各种供体细胞类型与受体心肌稳定且功能性耦合的程度方面的应用。
Although the adult mammalian myocardium exhibits a limited ability to undergo regenerative growth, its intrinsic renewal rate is insufficient to compensate for myocyte loss during cardiac disease. Transplantation of donor cardiomyocytes or cardiomyogenic stem cells is considered a promising strategy to reconstitute cardiac mass, provided the engrafted cells functionally integrate with host myocardium and actively contribute to its contractile force. We have previously developed a two-photon fluorescence microscopy-based assay that allows in situ screening of donor cell function following their intracardiac delivery. Here we review the techniques and summarize its application for quantitation of the extent to which a variety of donor cell types stably and functionally couple with the recipient myocardium.
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影响因子：
64.8
作者：
Murry, CE;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
DOI：
10.1111/j.1540-8167.1998.tb00110.x
发表时间：
1998-12-01
影响因子：
2.7
作者：
Biermann, M;Rubart, M;Zipes, DP
通讯作者：
Zipes, DP
影响因子：
12.4
作者：
Scherschel, John A.;Soonpaa, Mark H.;Rubart, Michael
通讯作者：
Rubart, Michael
影响因子：
2
作者：
Young PA;Clendenon SG;Byars JM;Decca RS;Dunn KW
通讯作者：
Dunn KW
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ