Surface modification of silicone elastomer with rosin acid-based quaternary ammonium salt for antimicrobial and biocompatible properties
Surface modification of silicone elastomer with rosin acid-based quaternary ammonium salt for antimicrobial and biocompatible properties
复制标题
采用松香酸基季铵盐对有机硅弹性体进行表面改性，以提高抗菌性和生物相容性
DOI：
10.1016/j.matdes.2020.108493
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发表时间：
2020-04-01
影响因子：
8.4
通讯作者：
Song, Zhanqian
中科院分区：
文献类型：
--
作者：
Li, Zhaoshuang;Liu, He;Song, Zhanqian
尽管有先进的灭菌和无菌技术，生物材料相关感染仍然对人类生命构成巨大威胁。因此，构建既具有高效抗菌活性又具有低细胞毒性的抗菌涂层具有重要意义。本文以马来海松酸季铵阳离子（MPA-N+）为基础，在硅橡胶（PDMS）表面成功地构建了抗菌涂层。通过扫描电镜（SEM）、能谱仪（EDS）和X射线光电子能谱仪（XPS）对改性PDMS（PDMS-g-MPA-N+）的表面形貌和化学组成进行了表征。PDMS-g-MPA-N+对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌、铜绿假单胞菌）菌株均具有优异的抗菌活性。此外，共聚焦激光扫描显微镜成像表明，它也可以有效地抑制细菌生物膜形成超过5天。最重要的是，PDMS-g-MPA-N+具有优异的生物相容性，对哺乳动物细胞没有明显的溶血和细胞毒性。该研究表明，用MPA-N+接枝的硅橡胶表面能够掺入可再生生物质，以有效地对抗细菌和生物膜的形成，用于生物医学应用。(C)2020年，任作家。爱思唯尔有限公司出版
Despite advanced sterilized and aseptic techniques, biomaterial-associated infections are still posing a great threat to human life. Therefore, constructing antimicrobial coating that exerts potent antimicrobial activity combined with low cytotoxicity is of great significance. Herein, an antimicrobial coating on the surface of silicone elastomer (PDMS) has been successful constructed, which based on maleopimaric acid quaternary ammonium cation (MPA-N+). The surface morphology and chemical composition of modified PDMS (PDMS-g-MPA-N+) were confirmed by scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), and X-ray photoelectron spectroscopy (XPS). The PDMS-g-MPA-N+ possess excellent antimicrobial activity against both Grampositive bacterial (Staphylococcus aureus) and Gram-negative bacterial (Escherichia coil, Pseudomonas aeruginosa) strains. In addition confocal laser scanning microscopy imaging demonstrated that it also can effectively inhibit bacterial biofilm formation over 5 days. Most importantly, PDMS-g-MPA-N+ exhibited excellent biocompatibility, which induced no significant hemolysis and cytotoxicity toward mammalian cells. The study demonstrated that the silicone elastomer surface grafted with MPA-N+ enabled the incorporation of a renewable biomass to effectively combat bacteria and biofilm formation for biomedical applications. (C) 2020 The Authors. Published by Elsevier Ltd.