The distribution of NCAM in the chick hindlimb during axon outgrowth and synaptogenesis.
The distribution of NCAM in the chick hindlimb during axon outgrowth and synaptogenesis.
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轴突生长和突触发生过程中 NCAM 在雏鸡后肢的分布。
DOI：
10.1016/0012-1606(86)90208-3
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发表时间：
1986
影响因子：
2.7
通讯作者：
Rutishauser,U
中科院分区：
文献类型：
--
作者：
Tosney,KW;Watanabe,M;Landmesser,L;Rutishauser,U
我们已经确定的分布和形式的神经细胞粘附分子（NCAM）在鸡后肢从最初的轴突生长（阶段17 - 1 - 2），直到3天posthatching免疫组织化学染色和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳免疫印迹。轴突染色强烈的NCAM在所有年龄段，而非神经元肢体组件染色表现出动态变化。神经管附近的硬结中的间充质细胞NCAM免疫反应呈前后顺序，与轴突生长的顺序相关。在推定的神经通路内和周围检测到低至中等量的NCAM，这与NCAM在轴突延伸中的允许作用一致，但与通路边界的精确划定不一致。在肌管上，NCAM的免疫反应性从第26阶段到第30阶段仍然很低，当它在neural和对照肢体中显著增加时，表明它的出现不是由神经依赖性活动或营养相互作用触发的。这种增加在时间上与肌肉分裂相关，并可能促进随后的轴突分支以及突触发生。染色保持高肌纤维在二级肌管形成和孵化前的一周，当多神经元神经支配撤回和成熟的突触模式变得稳定，只有下降。这种肌肉NCAM的损失首先发生在快肌纤维上，然后发生在慢肌纤维上。总之，这些结果表明，神经支配的时间可能是由肌肉控制，通过NCAM的表达，但随后的抑制肌肉NCAM可能会发生神经介导的活动的结果。
We have determined the distribution and form of the neural cell adhesion molecule (NCAM) in the chick hindlimb from initial axon outgrowth (stage 17 1 2) until 3 days posthatching by immunohistological staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis immunoblots. Axons stained intensely for NCAM at all ages, whereas non-neuronal limb components exhibited dynamic changes in staining. Mesenchymal cells in the sclerotome adjacent to the neural tube developed NCAM immunoreactivity in an anterior-posterior sequence which correlated with the sequence of axonal outgrowth. Low to moderate amounts of NCAM were detected within and surrounding presumptive nerve pathways, consistent with a permissive role for NCAM in axon extension, but not with a precise delineation of pathway boundaries. On myotubes immunoreactivity for NCAM remained low from stage 26 to 30 when it increased dramatically in both aneural and control limbs, indicating that its appearance is not triggered by nerve-dependent activity or trophic interactions. The increase was temporally associated with muscle cleavage and may encourage subsequent axon ramification as well as synaptogenesis. Staining remained high on muscle fibers during secondary myotube formation and only declined during the week before hatching when polyneuronal innervation is withdrawn and the mature synaptic pattern becomes stabilized. This loss of muscle NCAM occurred first on fast and then on slow muscle fibers. Together these results suggest that the timing of innervation may be controlled by the muscle, through NCAM expression, but that the subsequent suppression of muscle NCAM may occur as a result of nerve-mediated activity.