A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation
A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation
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关于小鼠切除伤口中多种生物标志物动力学的基础研究，用于伤口年龄估计。
DOI：
10.1016/j.jflm.2016.01.027
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发表时间：
2016
影响因子：
1.5
通讯作者：
Guan Da-Wei
中科院分区：
文献类型：
--
作者：
Wang Lin-Lin;Zhao Rui;Liu Chang-Sheng;Liu Min;Li Shan-Shan;Li Jiao-Yong;Jiang Shu-Kun;Zhang Miao;Tian Zhi-Ling;Wang Meng;Zhang Meng-Zhou;Guan Da-Wei
伤口年龄估计是法医实践中一个经典而又现代的主题。需要在不同类型的伤口上进行更多的实验，以进一步提高其准确性。本研究采用小鼠皮肤切除创面模拟皮肤缺损损伤。观察中性粒细胞和巨噬细胞的浸润、成纤维细胞和成纤维细胞的积累及其肌成纤维细胞的转化。此外，采用Western blotting和实时荧光定量PCR对IL-1β、IL-6、TNF-α、IFN-γ、MCP-1、CXCL12、VEGF-A、EGF、KGF、pro-col Iα2、pro-col IIIα1等创面愈合相关分子进行定量分析。中性粒细胞和巨噬细胞分别在12 h-1 d和3 d - 10 d在创面深度浸润。成纤维细胞和成纤维细胞从第3天开始在创面积累，并在第5天转化为可收缩的肌成纤维细胞。成纤维细胞和成纤维细胞的转化率分别在7 d - 10 d和10 d时最高（超过50%）。MCP-1 CXCL12从12 h增加到5 d,和il - 1β,肿瘤坏死因子-α和pro-col三世α1到7 d。il - 6和VEGF-A从12 h 1十佳d。增加pro-colα2从7到21 d。增加干扰素-γ减少从12 h 10 d。通过综合分析这些因素从形态学的角度来看,蛋白质和基因表达,这项研究为我们提供了基本的信息伤口年龄估计,特别是在伤口全层倒戈。
Wound age estimation is a classic but still modern theme in forensic practice. More experiments on different types of wound are needed to further improve its accuracy. In this study, mouse skin excisional wounds were created to simulate dermal defective injury. The neutrophil and macrophage infiltration, fibroblast and fibrocyte accumulation as well as their myofibroblastic transformation were examined. In addition, some wound healing-related molecules, including IL-1β, IL-6, TNF-α, IFN-γ, MCP-1, CXCL12, VEGF-A, EGF, KGF, pro-col Iα2 and pro-col IIIα1, were quantified by Western blotting and real-time quantitative PCR. Neutrophils and macrophages profoundly infiltrated in the wound at 12 h–1 d and 3 d–10 d respectively. Fibroblasts and fibrocytes accumulated in the wound from 3 d, and transformed into contractile myofibroblasts from 5 d post injury. The transformation ratios of fibroblasts and fibrocytes were highest at 7 d–10 d and 10 d respectively (over 50%). MCP-1 and CXCL12 increased from 12 h to 5 d, and IL-1β, TNF-α and pro-col IIIα1 up to 7 d. IL-6 and VEGF-A increased from 12 h to 1 d–10 d. Pro-col Iα2 increased from 7 d to 21 d. IFN-γ decreased from 12 h to 10 d. By comprehensive analysis of these factors from the perspective of morphometrics, protein and gene expressions, this study provided us with fundamental information for wound age estimation, especially in the wounds with full-thickness defection.