Preparation of a small intestinal submucosa modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating for pelvic reconstruction
Preparation of a small intestinal submucosa modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating for pelvic reconstruction
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DOI：
10.1177/0885328216628469
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Huang, Renshu
中科院分区：
文献类型：
--
作者：
Ge, Liangpeng;Liu, Lubin;Huang, Renshu
盆腔器官脱垂（POP）是影响许多成年女性的严重健康问题。POP患者的手术治疗包括使用支架材料重建脱垂骨盆的常见策略。然而，现有的骨盆重建材料在生物相容性、力学和免疫排斥方面不能满足临床要求。为了解决这些问题，选择聚丙烯（PP）补片，因为其具有较强的机械性能。采用小肠粘膜下层（SIS）通过贻贝启发的聚多巴胺涂层对PP补片进行改性，以增强其生物相容性。扫描电子显微镜（SEM）和原子力显微镜（AFM）结果表明，SIS成功地结合在PP网片表面。此外，细胞毒性结果表明，PP补片和SIS改性PP补片可安全使用。此外，体内试验证明，SIS改良PP补片组植入部位周围的纤维组织增生显著少于PP补片组。此外，免疫组织化学染色结果表明，在SIS改良PP补片组中，促炎性巨噬细胞（M1）的表达显著较低，促愈合性巨噬细胞（M2）的表达较高。ELISA检测表明，SIS改性PP网片组IL-1、IL-6的表达较PP网片组降低。这些结果表明，通过贻贝启发聚多巴胺涂层的SIS改性聚丙烯混合补片是骨盆重建的一种有前途的方法。
Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. Surgical treatments for POP patients comprise a common strategy in which scaffold materials are used to reconstruct the prolapsed pelvic. However, the existing materials for pelvic reconstruction cannot meet clinical requirements in terms of biocompatibility, mechanics and immunological rejection. To address these concerns, polypropylene (PP) mesh was selected because of its strong mechanical properties. Small intestinal submucosa (SIS) was used to modify the PP mesh via a mussel-inspired polydopamine coating to enhance its biocompatibility. The scanning electron microscopy (SEM) and atomic force microscopy (AFM) results demonstrated that SIS was successfully conjugated on the surface of the PP mesh. Moreover, the cytotoxicity results indicated that the PP mesh and SIS-modified PP mesh were safe to use. Furthermore, invivo tests demonstrated that the fibroplasia around the implanted site in the SIS-modified PP mesh group was significantly less than the fibroplasia around the PP mesh group. In addition, the immunohistochemistry staining results indicated that the expression of pro-inflammatory macrophages (M1) was substantially lower and that the expression of pro-healing macrophages (M2) was higher in the SIS-modified PP mesh group. Furthermore, ELISA detection indicated that the expression of IL-1 and IL-6 in the SIS-modified PP mesh group was reduced compared with the PP mesh group. These findings suggest that a SIS-modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating is a promising approach in pelvic reconstruction.