Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat
Effects of temperature on the diversity and community structure of known methanogenic groups and other archaea in high Arctic peat
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DOI：
10.1038/ismej.2007.84
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发表时间：
2008-01-01
期刊：
影响因子：
11
通讯作者：
Torsvik, Vigdis L.
中科院分区：
文献类型：
--
作者：
Hoj, Lone;Olsen, Rolf A.;Torsvik, Vigdis L.
在寒冷和温带的环境中有大量的食蟹猴种群，但对它们对气候变化引起的温度变化的潜在反应知之甚少。采用荧光原位杂交（FISH）、16 SrRNA基因克隆文库和变性梯度凝胶电泳（DGGE）相结合的方法，研究了温度对斯匹次卑尔根岛（Spitsbergen）弱酸性泥炭中古菌群落的影响。在低温（1和5摄氏度）下，活性古菌细胞的相对丰度较高（占总计数的11-12%），该群落主要由1.3b组Crenarchaeota和广古菌簇水稻簇V（RC-V）和Lake Dagow沉积物（LIDS）组成。温度升高降低了这些集群的多样性和相对丰度。泥浆中的产甲烷菌群是多样的，包括甲烷微生物目，甲烷杆菌，甲烷八叠球菌和甲烷菌的代表。产甲烷古菌的总体相对丰度和多样性随着温度的升高而增加，与甲烷生产率的强烈刺激一致。然而，DGGE分析表明，该群落的结构变化与温度和时间。虽然一些种群的相对丰度直接受温度的影响，但其他种群的相对丰度受间接影响或对温度没有反应。
Archaeal populations are abundant in cold and temperate environments, but little is known about their potential response to climate change-induced temperature changes. The effects of temperature on archaeal communities in unamended slurries of weakly acidic peat from Spitsbergen were studied using a combination of fluorescent in situ hybridization (FISH), 16S rRNA gene clone libraries and denaturing gradient gel electrophoresis (DGGE). A high relative abundance of active archaeall cells (11-12% of total count) was seen at low temperatures (1 and 5 degrees C), and this community was dominated by Group 1.3b Crenarchaeota and the euryarchaeal clusters rice cluster V (RC-V), and Lake Dagow sediment (LIDS). Increasing temperature reduced the diversity and relative abundance of these clusters. The methanogenic community in the slurries was diverse and included representatives of Methanomicrobiales, Methanobacterium, Methanosarcina and Methanosaeta. The overall relative abundance and diversity of the methanogenic archaea increased with increasing temperature, in accordance with a strong stimulation of methane production rates. However, DGGE profiling showed that the structure of this community changed with temperature and time. While the relative abundance of some populations was affected directly by temperature, the relative abundance of other populations was controlled by indirect effects or did not respond to temperature.