Stem cell factor is a chemotactic factor for human mast cells.
Stem cell factor is a chemotactic factor for human mast cells.
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DOI：
10.4049/jimmunol.153.8.3717
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发表时间：
1994-10
影响因子：
4.4
通讯作者：
G. Nilsson;J. Butterfield;Kenneth Nilsson;A. Siegbahn
中科院分区：
文献类型：
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作者：
G. Nilsson;J. Butterfield;Kenneth Nilsson;A. Siegbahn
肥大细胞是炎症反应的主要效应细胞之一，可以在全身大多数组织中找到。在炎症期间，可以看到肥大细胞数量增加，例如在引发过敏反应后的上皮内细胞层中。这种积累可能需要成熟肥大细胞或其前体的定向迁移。为了研究人类肥大细胞的迁移，我们使用人类肥大细胞系 HMC-1 和干细胞因子依赖性（也称为肥大细胞生长因子或 Kit 配体）脐带血来源的肥大细胞作为模型。结果表明，干细胞因子是体外人体肥大细胞的有效趋化因子。发现对 SCF 的趋化反应具有剂量依赖性，在 50 ng/ml 时达到最大值。 SCF 的活性可以被抗 SCF 抗体阻断。我们还测试了不同间分泌蛋白，即 IL-8、MIP-1 α、MIP-1 β、RANTES 和 MCAF（也称为单核细胞趋化蛋白 1）对人肥大细胞迁移的影响。只有 RANTES 对体外发育的肥大细胞具有趋化作用。所测试的间分泌均未诱导 HMC-1 细胞迁移。对于迁移，肥大细胞依赖于与细胞外基质蛋白的结合。因此，需要用纤连蛋白涂覆过滤器，而胶原蛋白或层粘连蛋白不会促进迁移。 HMC-1 细胞与纤连蛋白的粘附也可以在粘附测定中显示。此外，可以在肥大细胞表面检测到纤连蛋白受体的表达。这些结果表明，SCF不仅是体外人肥大细胞的生长和分化因子，而且还是此类细胞的有效趋化剂。
The mast cell is one of the major effector cells in inflammatory reactions and can be found in most tissues throughout the body. During inflammation, an increase in the number of mast cells can be seen, e.g., in the intraepithelial cell layer after a provoked allergic reaction. Such accumulation probably requires directed migration of mature mast cells or their precursors. To study the migration of human mast cells we used as a model the human mast cell line, HMC-1, and stem cell factor-dependent (also referred to as mast cell growth factor or Kit ligand) cord blood-derived mast cells. The results show that stem cell factor is a potent chemotactic factor for human mast cells in vitro. The chemotactic response to SCF was found to be dose dependent, reaching a maximum at 50 ng/ml. The activity of SCF could be blocked by anti-SCF Abs. We also tested the effect of different intercrines, i.e., IL-8, MIP-1 alpha, MIP-1 beta, RANTES, and MCAF (also referred to as monocyte chemotactic protein 1), on human mast cell migration. Only RANTES was chemotactic for in vitro-developed mast cells. None of the tested intercrines induced migration of HMC-1 cells. For migration, the mast cells were dependent on binding to an extracellular matrix protein. Thus, coating of the filters with fibronectin was required, whereas collagen or laminin did not promote migration. Adhesion of HMC-1 cells to fibronectin could also be shown in an adhesion assay. In addition, expression of receptors for fibronectin could be detected on the surface of the mast cells. These results show that SCF is not only a growth and differentiation factor for human mast cells in vitro but also a potent chemoattractant for such cells.