Tuning Trophoblast Motility in a Gelatin Hydrogel via Soluble Cues from the Maternal-Fetal Interface.
Tuning Trophoblast Motility in a Gelatin Hydrogel via Soluble Cues from the Maternal-Fetal Interface.
复制标题
通过母胎界面的可溶性线索调节明胶水凝胶中的滋养层运动性。
DOI：
10.1089/ten.tea.2020.0097
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发表时间：
2021
影响因子：
--
通讯作者：
Harley,BrendanAC
中科院分区：
文献类型：
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作者：
Zambuto,SamanthaG;Clancy,KathrynBH;Harley,BrendanAC
滋养层细胞在妊娠中起着多种关键作用，特别是调节胚泡与子宫内膜的附着，以及侵入子宫内膜并积极重塑子宫内膜以促进生长胚胎的生物运输需求。尽管滋养层细胞的侵入过程中的重要性，在怀孕的早期阶段必不可少的，很多仍然是未知的信号分子的平衡，可能会影响滋养层细胞侵入子宫内膜。本研究的目的是使用三维滋养层球体运动分析，以检查从母胎界面的提示滋养层运动的影响。我们报告使用甲基丙烯酰胺功能化的明胶水凝胶，以支持定量分析滋养层生长面积和细胞活力。我们发现，这种滋养层运动的多维模型可以解决在已知的侵袭促进剂，表皮生长因子和已知的侵袭抑制剂，转化生长因子β1存在下，生长面积和活力的量化差异。然后，我们调查的敏感性滋养层运动皮质醇，激素与外源性应激。总之，这种方法提供了一个工具集，以调查协调行动的生理和病理生理过程的早期阶段的滋养层细胞入侵。
Trophoblast cells play multiple critical roles in pregnancy, notably modulating blastocyst attachment to the endometrium as well as invading into and actively remodeling the endometrium to facilitate biotransport needs of the growing embryo. Despite the importance of trophoblast invasion for processes essential at early stages of pregnancy, much remains unknown regarding the balance of signaling molecules that may influence trophoblast invasion into the endometrium. The goal of this study was to use three-dimensional trophoblast spheroid motility assays to examine the effect of cues from the maternal–fetal interface on trophoblast motility. We report use of a methacrylamide-functionalized gelatin hydrogel to support quantitative analysis of trophoblast outgrowth area and cell viability. We show that this multidimensional model of trophoblast motility can resolve quantifiable differences in outgrowth area and viability in the presence of a known invasion promoter, epidermal growth factor, and a known invasion inhibitor, transforming growth factor β1. We then investigate the sensitivity of trophoblast motility to cortisol, a hormone associated with exogenous stressors. Together, this approach provides a toolset to investigate the coordinated action of physiological and pathophysiological processes on early stages of trophoblast invasion.