DIFFERENTIATION OF DOPAMINERGIC AND NORADRENERGIC NEURONS IN RAT SPINAL-CORD
DIFFERENTIATION OF DOPAMINERGIC AND NORADRENERGIC NEURONS IN RAT SPINAL-CORD
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DOI：
10.1111/j.1471-4159.1978.tb12403.x
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发表时间：
1978-01-01
影响因子：
4.7
通讯作者：
NEFF, NH
中科院分区：
文献类型：
--
作者：
COMMISSIONG, JW;GALLI, CL;NEFF, NH
用气相色谱-质谱法测定大鼠脊髓和小脑不同部位去甲肾上腺素（NE）、多巴胺（DM）和3-甲氧基-4-羟基苯乙酸（HVA）的含量。在小脑，其中不包含多巴胺能神经元，NE DM含量的比例为47，而在脊髓的部分，这一比例在11和19之间变化。在脊髓中，地昔帕明（25 mg/kg，i. p.）加上6-羟基多巴胺（6-HDA，脑池内100 μ g），DM显着消耗，但NE没有显着消耗。相反，在苯托品（25 mg/kg，i. p.）加6-HDA后，NE明显减少，但DM无明显减少。氯丙嗪（10 mg/kg，i. p.）或氯氮平（25 mg/kg，i. p.）在处理后1小时引起脊髓HVA浓度的显著增加。在脊髓中测得的HVA增加显然不是源于大脑。电毁损蓝斑后NE明显降低，但DM无明显变化。利血平对脊髓DM和NE的消耗呈剂量依赖性，DM消耗的阈值剂量小于NE消耗的阈值剂量。在T10 [胸椎-第10椎骨]脊髓横断后7天，腰椎区域的脊髓DM显著减少。大鼠脊髓中可能存在独立于去甲肾上腺素能神经元的多巴胺能神经元，且下行轴突终末可能存在多巴胺能神经元。
Norepinephrine (NE), dopamine (DM) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) content were measured in different parts of rat spinal cord and cerebellum by a gas chromatographic-mass spectrometric method. In cerebellum, which does not contain dopaminergic neurons, the ratio of NE to DM content was 47, whereas in parts of the spinal cord this ratio varied between 11 and 19. In the cord after desipramine (25 mg/kg, i.p.) plus 6-hydroxydopamine (6-HDA, 100 .mu.g intracisternally), there was a significant depletion of DM but not of NE. Conversely, after benztropine (25 mg/kg, i.p.) plus 6-HDA there was a significant depletion of NE but not of DM. Chlorpromazine (10 mg/kg, i.p.) or clozapine (25 mg/kg, i.p.) caused a significant increase in spinal cord HVA concentration 1 h after treatment. The increased HVA measured in the cord apparently did not originate in the brain. After electrolytic lesion of the locus coeruleus there was a significant reduction of NE but not of DM. Spinal cord DM and NE were depleted by reserpine in a dose-dependent manner, the threshold dose for DM depletion being less than that for NE depletion. Seven days after cord transection at T10 [thoracic-10th vertebrae] spinal cord DM was significantly reduced in the lumbar region. Dopaminergic neurons may exist in rat spinal cord independently of noradrenergic neurons, and the DM is likely to be present in the terminals of descending axons.