Cell specificity in the formation of multinucleated striated muscle.
Cell specificity in the formation of multinucleated striated muscle.
复制标题
多核横纹肌形成中的细胞特异性。
DOI：
10.1016/0014-4827(69)90218-3
复制
发表时间：
1969
影响因子：
3.7
通讯作者：
D. Maslow
中科院分区：
文献类型：
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作者：
D. Maslow
将新鲜分离的鸡胚肌源性细胞与小鼠胚胎肌细胞以及未处理和放线菌素D处理的小鼠胚胎肝细胞一起培养。使用放射自显影和组织学染色检查这些培养物中发育的肌带以确定细胞类型，证实来自不同生物体的肌细胞被并入常见的多核肌肉单位中，但排除非肌细胞。然而，放线菌素D处理的肝细胞被掺入。在这些细胞中的RNA和蛋白质合成的分析表明，尿苷掺入的快速和完全停止和氨基酸掺入的几乎完全停止。这些结果表明存在一个识别过程，该过程受到正常RNA和蛋白质合成机制破坏的干扰。在细胞表面的识别因子的建议。
Freshly disaggregated embryonic chick myogenic cells were cultured together with embryonic mouse muscle cells and with both untreated and actinomycin D treated mouse embryonic liver cells. Examination of muscle straps which developed in these cultures using autoradiography and histological staining to determine cell type confirmed that muscle cells from different organisms are incorporated into common multinucleated muscle units, but that non-muscle cells are excluded. Actinomycin D treated liver cells, however, are incorporated. The analysis of RNA and protein synthesis in these cells indicated a rapid and complete cessation of uridine incorporation and an almost complete cessation of amino acid incorporation. These results suggest the existence of a recognition process which is disturbed by the disruption of normal RNA and protein synthesis mechanism. A recognition factor at the cell surface is proposed.