Potential biomarkers of acute cerebral infarction detected by SELDI-TOF-MS
Potential biomarkers of acute cerebral infarction detected by SELDI-TOF-MS
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SELDI-TOF-MS检测急性脑梗死的潜在生物标志物
DOI：
10.1309/ca242r5vy14huge8
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发表时间：
2008-08-01
影响因子：
3.5
通讯作者：
Song, Shanjun
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoping;Guo, Tao;Song, Shanjun
没有单一的生物标志物用于急性脑梗死的常规诊断。我们筛选了 92 份血浆样本中的潜在生物标志物，其中包括 32 名急性脑梗死患者和 60 名医院对照受试者的样本。使用 SELDI-TOF-MS (Ciphergen Biosystems, Fremont, CA) 分析预处理的血浆样品。通过将等离子体应用于弱阳离子交换（CM10 ProteinChip，Ciphergen Biosystems）阵列来生成质荷比（m/z）的蛋白质组谱。选择由 13 种生物标志物组成的差异模式，基于它们对急性脑梗塞患者和对照受试者之间最佳分离的集体贡献，敏感性分别为 84.4%，特异性为 95.0%。使用 SELDI-TOF-MS 和 ProteinChip 技术进行血浆蛋白质组分析显示出区分急性脑梗死患者和对照受试者的潜力。急性脑梗塞的诊断可能应该取决于一组生物标志物的使用。
No single biologic marker is used in the routine diagnosis of acute cerebral infarction. We screened for potential biomarkers in 92 plasma samples, including samples from 32 patients with acute cerebral infarction and 60 hospital control subjects. Pretreated plasma samples were analyzed using SELDI-TOF-MS (Ciphergen Biosystems, Fremont, CA). Proteomic spectra of mass to charge ratio (m/z) were generated by the application of plasma to weak cation exchange (CM10 ProteinChip, Ciphergen Biosystems) arrays. A differential pattern consisting of 13 biomarkers was selected based on their collective contribution to the optimal separation between patients with acute cerebral infarction and control subjects with a sensitivity of 84.4% and specificity of 95.0%, respectively. Plasma proteomic profiling with SELDI-TOF-MS and ProteinChip technologies shows potential in discriminating patients with acute cerebral infarction and control subjects. Diagnosis of acute cerebral infarction should probably depend on the use of a panel of biomarkers.