High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array ultraviolet detection in the identification of flavonol aglycones and glycosides in berries.
High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array ultraviolet detection in the identification of flavonol aglycones and glycosides in berries.
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DOI：
10.1016/s0021-9673(98)00756-0
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发表时间：
1998-12
期刊：
影响因子：
--
通讯作者：
Sari Häkkinen;Seppo Auriola
中科院分区：
文献类型：
--
作者：
Sari Häkkinen;Seppo Auriola
采用高效液相色谱-电喷雾-质谱法（HPLC-ESI-MS）研究了浆果中黄酮醇苷元和苷类成分。此外，还采用光电二极管阵列检测（DAD）进行苷元的鉴定。HPLC-ESI-MS技术在鉴定浆果提取物中黄酮醇苷元和苷类化合物方面具有重要的应用价值。这种电离技术提供了苷元和糖苷的结构信息，而无需耗时的预纯化或衍生化步骤。槲皮素苷元经ESI-MS和DAD鉴定。两种方法均从3种浆果中鉴定出杨梅素苷元。槲皮素的己糖、脱氧己糖和戊糖衍生物是含量最多的黄酮醇苷类。在黑加仑中鉴定出杨梅素的两种苷和山奈酚的一种苷。为了证实HPLC-ESI-MS程序获得的数据，从四种浆果中分离苷，水解，硅烷化，并使用气相色谱-质谱分析糖。
High-performance liquid chromatography–electrospray ionization-mass spectrometry (HPLC–ESI-MS) was used to study flavonol aglycones and glycosides in berries. For the identification of aglycones, photodiode-array detection (DAD) was also used. The HPLC–ESI-MS technique is highly valuable in the identification of flavonol aglycones and glycosides from berry extracts. This ionization technique provides information on the structure of the aglycones and glycosides without time-consuming pre-purification or derivatization steps. Quercetin aglycone was identified with both ESI-MS and DAD in all of the berries studied. Myricetin aglycone was identified with both techniques in three berries. Hexose, deoxyhexose–hexose and pentose derivatives of quercetin were the most abundant flavonol glycosides identified. Two glycosides of myricetin and one glycoside of kaempferol were identified in blackcurrant. To confirm the data obtained using the HPLC–ESI-MS procedure, fractions of the glycosides from four berries were separated, hydrolyzed, silylated and the sugars were analyzed using gas chromatography–mass spectrometry.