Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.
Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.
复制标题
DOI：
10.1016/j.celrep.2015.07.015
复制
发表时间：
2015-08-18
期刊：
影响因子：
8.8
通讯作者：
Capel B
中科院分区：
文献类型：
--
作者：
DeFalco T;Potter SJ;Williams AV;Waller B;Kan MJ;Capel B
睾丸通过平衡精原干细胞(SSCs)的自我更新和分化，在整个男性生殖寿命中产生精子。SSC生态位的一部分被认为位于睾丸生精小管外；然而，调节精原细胞分裂和分化的特定间质成分仍未确定。我们鉴定了不同群体的睾丸巨噬细胞，其中一种位于生精小管表面，与富含未分化精原细胞的小管区接近。这些巨噬细胞表达精原细胞增殖和分化诱导因子，如集落刺激因子1(CSF1)和参与维甲酸(RA)生物合成的酶。我们发现，巨噬细胞的一过性耗尽会导致精原细胞分化的中断。这些发现揭示了巨噬细胞在睾丸精原细胞巢中的一个意想不到的作用，并增加了巨噬细胞在其他组织的干细胞/祖细胞调节中发挥以前未被认识到的作用的可能性。
The testis produces sperm throughout the male reproductive lifespan by balancing self-renewal and differentiation of spermatogonial stem cells (SSCs). Part of the SSC niche is thought to lie outside the seminiferous tubules of the testis; however, specific interstitial components of the niche that regulate spermatogonial divisions and differentiation remain undefined. We identified distinct populations of testicular macrophages, one of which lies on the surface of seminiferous tubules in close apposition to areas of tubules enriched for undifferentiated spermatogonia. These macrophages express spermatogonial proliferation- and differentiation-inducing factors, such as colony stimulating factor 1 (CSF1) and enzymes involved in retinoic acid (RA) biosynthesis. We show that transient depletion of macrophages leads to a disruption in spermatogonial differentiation. These findings reveal an unexpected role for macrophages in the spermatogonial niche in the testis, and raise the possibility that macrophages play previously unappreciated roles in stem/progenitor cell regulation in other tissues.