Location of P element insertions in the proximal promoter region of Hsp70A is consequential for gene expression and correlated with fecundity in Drosophila melanogaster
Location of P element insertions in the proximal promoter region of Hsp70A is consequential for gene expression and correlated with fecundity in Drosophila melanogaster
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DOI：
10.1007/s12192-007-0002-4
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发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Feder, Martin E.
中科院分区：
文献类型：
--
作者：
Chen, Bing;Shilova, Victoria Y.;Feder, Martin E.
我们比较了一系列在Hsp 70 A基因转录起始位点5'端-28 ~-144个核苷酸处插入P元件的果蝇品系，以阐明插入位点对Hsp 70 A基因表达的影响。虽然所有的插入减少低于对照菌株的Hsp 70 A的表达，减少的幅度是负相关的转录起始位点和插入位点之间的核苷酸的数量。一个预先存在的假设是，在热休克蛋白启动子中天然存在的转座因子插入在某些情况下可能是有益的，这可能是它们在自然群体中保留的原因。在本研究中，在一个控制线热休克降低生育力，而在与P元素插入线热休克通常增加生育力。最后，根据簇特异性定量RT-PCR，Hsp 70 A簇基因的表达通常大于Hsp 70 B基因簇基因的表达，尽管后者数量更多，并且在这种情况下，不含P元件插入。
We compared a series of Drosophila strains with P element insertions from -28 to -144 nucleotides 5' to the transcription start site of the Hsp70A genes-corresponding to the range of naturally occurring P element insertion sites-to elucidate the consequences of insertion site for Hsp70A gene expression. Although all insertions reduced Hsp70A expression below that of a control strain, the magnitude of the reduction was inversely related to the number of nucleotides between the transcription start site and the insertion site. A pre-existing hypothesis is that naturally occuring transposable element insertions in Hsp promoters may be beneficial in some circumstances, which may account for their retention in natural populations. In the present study, in a control line heat shock reduced fecundity, whereas in lines with P element insertions heat shock typically increased fecundity. Finally, according to cluster-specific quantitative RT-PCR, expression of the Hsp70A cluster genes was typically greater than that of the Hsp70B gene cluster genes, although the latter are more numerous and, in this case, free of P element insertions.