Identification of the genes that are expressed in the upper layers of the neocortex.
Identification of the genes that are expressed in the upper layers of the neocortex.
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DOI：
10.1093/cercor/bhh074
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发表时间：
2004-10
期刊：
影响因子：
3.7
通讯作者：
Yun Zhong;M. Takemoto;Tsuyoshi Fukuda;Yuki Hattori;F. Murakami;D. Nakajima;M. Nakayama;N. Yamamoto
中科院分区：
文献类型：
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作者：
Yun Zhong;M. Takemoto;Tsuyoshi Fukuda;Yuki Hattori;F. Murakami;D. Nakajima;M. Nakayama;N. Yamamoto
层状特异性是新皮质回路最显着的特征之一。为了探索这种特异性的分子基础，特别是与丘脑皮质连接性相关的分子基础，我们通过构建消减 cDNA 文库来搜索在上皮质层中表达的基因，该文库富集了在围产期大鼠体感皮层第 4 层中表达的基因。差异筛选、序列分析和原位杂交表明，新的 unc5 家族成员 (unc5h4)、deltex 样基因、干细胞因子 (SCF) 和肌细胞特异性增强因子 2C (MEF-2C) 在出生后第 7 天的第 4 层或第 2/3-4 层中特异性表达，此时层状组织和基本皮质回路已经建立。就区域特异性而言，unc5h4和SCF信号在感觉皮层中较强，而MEF-2C和deltex-like基因在所有新皮层区域中表达相当均匀。发育过程中的分析表明，这些基因的表达在胚胎晚期和出生后早期发育阶段之间很明显，但 MEF-2C 表达除外，该表达在后期阶段持续存在。这些结果表明，某些类型的分子，包括转录因子、受体和配体分子，在发育中的新皮质的上层中特异性表达，表明在皮质细胞和电路的层状规范中发挥作用。
Laminar specificity is one of the most striking features of neocortical circuitry. To explore the molecular basis of this specificity, particularly in relation to thalamocortical connectivity, we searched for the genes expressed in the upper cortical layers by constructing a subtraction cDNA library that was enriched for genes expressed in layer 4 of perinatal rat somatosensory cortex. Differential screening, sequence analysis and in situ hybridization demonstrated that a new unc5 family member (unc5h4), deltex-like gene, stem cell factor (SCF) and myocyte-specific enhancer factor-2C (MEF-2C) were specifically expressed in layer 4 or layers 2/3-4 at postnatal day 7, by when laminar organization and fundamental cortical circuitries have been established. In terms of regional specificity, unc5h4 and SCF signals were stronger in sensory cortices, whereas MEF-2C and deltex-like gene were expressed rather uniformly in all neocortical regions. Analysis during development demonstrated that expression of these genes was pronounced between late embryonic and early postnatal developmental stages, except for MEF-2C expression, which continued in later stages. These results demonstrate that certain types of molecules including transcription factors, receptor and ligand molecules, are expressed specifically in the upper layers of the developing neocortex, suggesting a role in laminar specification of cortical cells and circuitry.