Urate and p-aminohippurate transport in rat renal basolateral vesicles.
Urate and p-aminohippurate transport in rat renal basolateral vesicles.
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大鼠肾基底外侧囊泡中尿酸盐和对氨基马尿酸盐的转运。
DOI：
10.1152/ajprenal.1985.249.5.f654
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
E. Weinman
中科院分区：
文献类型：
--
作者：
A. Kahn;H. Shelat;E. Weinman
我们研究了尿酸盐在大鼠肾脏基底外侧膜囊泡中的转运，并确定了尿酸盐和对氨基马尿酸盐（PAH）转运之间的关系。尿酸盐未转化为尿囊素，尿酸盐的摄取代表转运至具有促炎活性的囊内空间。2.4丙磺舒、2.4 DIDS和1.4未标记尿酸盐分别抑制基底外侧囊泡中53 μ M [14 C]尿酸盐的10 s摄取39 +/- 6、49 +/- 10和35 +/- 3%（以mM计）。通过用1.5 mM未标记的尿酸盐预加载基底外侧囊泡，353 μ M [14 C]尿酸盐的10 s摄取被反式刺激82 +/- 8%。尿酸盐的摄取通过Cl-的向外定向梯度（Cl-in = 25 mM，Cl-out = 5 mM）来刺激。这种效果是不是随之而来的一个更正电性的囊内空间，监测电压敏感的钠-L-苹果酸共转运系统。基底外侧囊泡中Cl-梯度刺激的尿酸盐摄取组分不被4.8 mM PAH顺式抑制，而刷状缘囊泡中Cl-梯度刺激的尿酸盐摄取被PAH顺式抑制43 +/- 5%。在没有Cl-的情况下，4.8 mM PAH没有顺式抑制，5.4 mM PAH或6.4 mM乳酸盐没有反式刺激基底外侧囊泡中尿酸盐的摄取，与刷状缘囊泡获得的结果形成对比。基底外侧囊泡中尿酸盐的摄取不受外部Na+相对于K+、Li+或Cs+的刺激。相比之下，基底外侧囊泡中的PAH摄取被外部Na+刺激87 +/- 9%。（250字处删节）
We examined the transport of urate in basolateral membrane vesicles from the rat kidney and determined the relationship between the transport of urate and p-aminohippurate (PAH). Urate was not converted to allantoin, and the uptake of urate represented transport into an osmotically active intravesicular space. The 10-s uptake of 53 microM [14C]urate in basolateral vesicles was inhibited 39 +/- 6, 49 +/- 10, and 35 +/- 3% by (in mM) external 2.4 probenecid, 2.4 DIDS, and 1.4 unlabeled urate, respectively. The 10-s uptake of 353 microM [14C]urate was trans-stimulated 82 +/- 8% by preloading basolateral vesicles with 1.5 mM unlabeled urate. The uptake of urate was stimulated by an outwardly directed gradient for Cl- (Cl-in = 25 mM, Cl-out = 5 mM). This effect was not consequent to a more electropositive intravesicular space, as monitored by the voltage-sensitive sodium-L-malate cotransport system. The Cl- gradient-stimulated component of urate uptake in basolateral vesicles was not cis-inhibited by 4.8 mM PAH, whereas Cl gradient-stimulated urate uptake in brush border vesicles was cis-inhibited 43 +/- 5% by PAH. In the absence of Cl-, 4.8 mM PAH did not cis-inhibit, and 5.4 mM PAH or 6.4 mM lactate did not trans-stimulate the uptake of urate in basolateral vesicles, contrasting with results obtained with brush border vesicles. The uptake of urate in basolateral vesicles was not stimulated by external Na+ relative to K+, Li+, or Cs+. In contrast, PAH uptake in basolateral vesicles was stimulated 87 +/- 9% by external Na+.(ABSTRACT TRUNCATED AT 250 WORDS)