Biomarkers of exposure to tobacco smoke and environmental pollutants in mothers and their transplacental transfer to the foetus. Part II. Oxidative damage
Biomarkers of exposure to tobacco smoke and environmental pollutants in mothers and their transplacental transfer to the foetus. Part II. Oxidative damage
复制标题
DOI：
10.1016/j.mrfmmm.2009.04.010
复制
发表时间：
2009-10-02
影响因子：
2.3
通讯作者：
Sram, R. J.
中科院分区：
文献类型：
--
作者：
Rossner, P., Jr.;Milcova, A.;Sram, R. J.
对大分子的氧化损伤可能会对健康产生许多负面影响。我们测量了80名新生儿和79名母亲的DNA，蛋白质和脂质的氧化损伤，分析了母亲的烟草烟雾暴露对氧化应激的影响，并评估了氧化应激标志物和大体积和PAH（多环芳烃）特异性DNA加合物之间的相关性。平均水平（+/-S.D.）胎盘中每10（5）dG的8-氧代脱氧鸟苷（8-oxodG）水平为2.85 +/- 0.78;我们没有看到暴露于烟草烟雾和未暴露于烟草烟雾的母亲所生新生儿的8-oxodG水平之间的差异。蛋白质羰基水平。一种蛋白质氧化的标志物，在脐带和母体静脉血浆中是相当的（新生儿和母亲的血浆分别为17.4 ± 3.2和17.6 ± 4.2 nmol/ml，p = 0.66）。以血浆中15-F（2 t）-异前列烷（15-F（2 t）-IsoP）水平测量的脂质过氧化在新生儿中显著高于母亲（新生儿和母亲分别为362 +/-129和252 +/-130 pg/ml，p < 0.001）。脐血中蛋白质羰基化和15-F（2 t）-IsoP水平与母血中的蛋白质羰基化和15-F（2 t）-IsoP水平显著相关（p < 0.001）。8-oxodG水平与脐带血浆中的血浆羰基以及母体血浆中的可替宁水平（烟草烟雾暴露的标志物）正相关。8-oxodG水平还与新生儿和母亲淋巴细胞DNA中的大体积DNA加合物以及胎盘中的PAH-DNA加合物相关。我们的研究结果表明，新生儿的脂质过氧化作用高于母亲，新生儿和母亲之间的氧化应激标记物分析密切相关，氧化应激和DNA加合物的诱导之间的关系。(C)2009 Elsevier B. V.版权所有
Oxidative damage to macromolecules may have numerous negative health consequences. We measured oxidative damage to DNA, proteins and lipids in 80 newborns and 79 mothers, analyzed the effect of mother's tobacco smoke exposure on oxidative stress, and assessed correlations between oxidative stress markers and bulky and PAH (polycyclic aromatic hydrocarbons)-specific DNA adducts. Mean levels (+/-S.D.) of 8-oxodeoxyguanosine (8-oxodG) per 10(5) dG in the placenta were 2.85 +/- 0.78; we did not see a difference between 8-oxodG levels in newborns born to mothers exposed and unexposed to tobacco smoke. Protein carbonyl levels. a marker of protein oxidation, were comparable in the umbilical cord and in maternal venous blood plasma (17.4 +/- 3.2 and 17.6 +/- 4.2 nmol/ml plasma in newborns and mothers, respectively, p = 0.66). Lipid peroxidation measured as levels of 15-F(2t)-isoprostane (15-F(2t)-IsoP) in plasma was significantly higher in newborns than in mothers (362 +/- 129 and 252 +/- 130 pg/ml in newborns and mothers, respectively, p < 0.001) We did not find any effect of tobacco smoke exposure on either biomarker in any group. Levels of both protein carbonyls and 15-F(2t)-IsoP in cord blood significantly correlated with those in maternal plasma (p < 0.001). 8-oxodG levels positively correlated with plasma carbonyls in cord plasma, as well as with cotinine levels (marker of tobacco smoke exposure) in maternal plasma. 8-oxodG levels also correlated with bulky DNA adducts in lymphocyte DNA of newborns and mothers and with PAH-DNA adducts in the placenta. Our results showed higher lipid peroxidation in newborns than in mothers, close correlation of analyzed oxidative stress markers between newborns and mothers, and a relationship between oxidative stress and induction of DNA adducts. (C) 2009 Elsevier B.V. All rights reserved