Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.
Amino acid sequence of the Fv region of a human monoclonal IgM (protein WEA) with antibody activity against 3,4-pyruvylated galactose in Klebsiella polysaccharides K30 and K33.
复制标题
人单克隆 IgM（蛋白质 WEA）Fv 区的氨基酸序列，具有针对克雷伯氏菌多糖 K30 和 K33 中 3,4-丙酮酰化半乳糖的抗体活性。
DOI：
10.1073/pnas.80.15.4837
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发表时间：
1983
影响因子：
11.1
通讯作者：
Frangione,B
中科院分区：
文献类型：
--
作者：
Goñi,F;Frangione,B
我们已经确定了从患有Waldenström巨球蛋白血症的WEA患者血浆中分离的具有3,4-丙酮酰化半乳糖抗体活性的人单克隆IgM-kappa的Fv[可变重（VH）和可变轻（VL）]区域的氨基酸序列。VH区有114个残基，属于III亚群，有一个非常短的第三互补决定区（CDR3），可能是由于D段较小或D-J重排（D, diversity； J, joining）。VL区有108个残基，属于V kappa I亚群。与人类VHIII和V kappa I家族的其他成员相比，WEA Fv在框架残基内似乎没有显著差异，但具有独特的cdr，可能负责特定的抗体活性。另一种IgM-kappa （GAL）具有尚未确定的抗体活性，与WEA在V kappa中具有惊人的同源性，包括相同的CDR1。
We have determined the amino acid sequence of the Fv [variable heavy (VH) and variable light (VL)] region of a human monoclonal IgM-kappa with antibody activity against 3,4-pyruvylated galactose, isolated from the plasma of patient WEA with Waldenström macroglobulinemia. The VH region has 114 residues, belongs to subgroup III, and has a very short third complementarity-determining region (CDR3), probably due to a small D segment/or an unusual D-J rearrangement (D, diversity; J, joining). The VL region has 108 residues and belongs to subgroup V kappa I. Compared to other members of the human VHIII and V kappa I families, WEA Fv does not appear to have significant differences within the framework residues but has unique CDRs that might be responsible for the particular antibody activity. Another IgM-kappa (GAL), which has an as-yet-undetermined antibody activity, shares a striking homology in V kappa with WEA, including an identical CDR1.