Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic
Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic
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DOI：
10.1007/s00436-010-1929-4
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发表时间：
2010-09-01
影响因子：
2
通讯作者：
Literak, Ivan
中科院分区：
文献类型：
--
作者：
Bartova, Eva;Sedlak, Kamil;Literak, Ivan
2007年1月，从捷克共和国9个不同地区的552匹健康马身上采集了血液样本。用竞争抑制酶联免疫吸附试验检测血清中的犬新孢子虫抗体，并用间接荧光抗体试验进行确认。用乳胶凝集试验检测同一样本的血清弓形虫抗体。在竞争抑制酶联免疫吸附试验中，552匹马中有131匹(24%)对新孢子虫抗体呈阳性反应；其中7匹马的抑制率为千分之50。间接荧光试验证实，只有两份样品呈阳性，最终滴度分别为50和100，其余样品均为阴性。125匹马(23%)检出弓形虫抗体。这是新孢子菌的首次血清学调查。在捷克共和国的马身上进行了抗体试验。
During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had a parts per thousand yen50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.