Ex vivo peripheral nerve detection of rats by spontaneous Raman spectroscopy.
Ex vivo peripheral nerve detection of rats by spontaneous Raman spectroscopy.
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DOI：
10.1038/srep17165
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发表时间：
2015-11-25
影响因子：
4.6
通讯作者：
Takamatsu T
中科院分区：
文献类型：
--
作者：
Minamikawa T;Harada Y;Takamatsu T
神经保留手术越来越多地被应用于避免手术后肢体和器官的功能缺陷。然而，由于形状和颜色与非神经组织相似，应该保留的外周神经有时会被错误识别。为了避免周围神经的错误识别，需要开发原位神经检测方法。在这项研究中，我们报告了无标记检测离体周围神经的Wistar大鼠使用拉曼光谱。我们获得了Wistar大鼠周围神经（有髓神经和无髓神经）及其邻近组织的拉曼光谱，无需任何处理，如固定和/或染色。为了识别组织种类和进一步分析光谱特征，我们提出了一种基于主成分回归的判别分析与周围神经及其邻近组织的代表性拉曼光谱。模型对Wistar大鼠有髓神经和无髓神经的特异性分别为93.5%和99.4%，敏感性分别为88.3%和95.5%。此外，重要的光谱特征，用于组织物种的鉴定，通过详细的主成分分析的代表性拉曼光谱的组织。我们所提出的方法可能会提供一个独特的和强大的工具，周围神经检测神经保留手术在未来。
Nerve-sparing surgery is increasingly being applied to avoid functional deficits of the limbs and organs following surgery. Peripheral nerves that should be preserved are, however, sometimes misidentified due to similarity of shape and color to non-nerve tissues. To avoid misidentification of peripheral nerves, development of an in situ nerve detection method is desired. In this study, we report the label-free detection of ex vivo peripheral nerves of Wistar rats by using Raman spectroscopy. We obtained Raman spectra of peripheral nerves (myelinated and unmyelinated nerves) and their adjacent tissues of Wistar rats without any treatment such as fixation and/or staining. For the identification of tissue species and further analysis of spectral features, we proposed a principal component regression-based discriminant analysis with representative Raman spectra of peripheral nerves and their adjacent tissues. Our prediction model selectively detected myelinated nerves and unmyelinated nerves of Wistar rats with respective sensitivities of 95.5% and 88.3% and specificities of 99.4% and 93.5%. Furthermore, important spectral features for the identification of tissue species were revealed by detailed analysis of principal components of representative Raman spectra of tissues. Our proposed approach may provide a unique and powerful tool for peripheral nerve detection for nerve-sparing surgery in the future.