Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay.
Diagnosis of drug allergy by the lymphocyte stimulation test with the MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay.
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通过 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑]测定法进行淋巴细胞刺激试验诊断药物过敏。
DOI：
10.1248/bpb.16.686
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发表时间：
1993
影响因子：
2
通讯作者：
H. Nagai
中科院分区：
文献类型：
--
作者：
T. Yoshimura;C. Kurita;M. Hayata;H. Nagai
淋巴细胞刺激试验（LST）是鉴定对特定药物过敏的最有用的实验室检查之一。本研究使用[3-（4，5-二甲基噻唑-2-基）-2，5-二苯基溴化四氮唑]（MTT）测定来检查LST作为诊断药物过敏的方法的效用。采用IL-2依赖的人T淋巴瘤CTLL细胞和人外周血淋巴细胞，确定了MTT法的基本实验条件。所需MTT量为0.5mg/孔，MTT甲瓒峰最大值接近565 nm。淋巴细胞的胚细胞生成表示为刺激指数（SI）。在细胞数量范围为5 × 10（5）至1 × 10（6）个细胞/ml时，SI值几乎恒定。用正常人外周血淋巴细胞测定某些药物的SI小于1.3。应用MTT法对43例可疑药物性肝炎患者的83种药物进行了过敏性试验。SI的范围为0.92-2.02。在62.8%的病例中观察到SI ≥ 1.2，在51.2%的病例中观察到SI ≥ 1.3，在41.9%的病例中观察到SI ≥ 1.4。有26种药物的SI大于1.3。其中7种是抗生素，其余包括抗高血压药、镇痛药、精神药物、抗过敏药和抗癫痫药。该方法非常适合于临床情况下药物过敏的诊断。MTT法的最大好处是可以在普通实验室进行完整的测试程序。
The lymphocyte stimulation test (LST) is one of the most useful laboratory tests for the identification of allergy to a specific drug. The present study was conducted to examine utility of the LST using the [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay as a method for diagnosing drug allergy. The basic experimental conditions for the MTT assay were determined by using CTLL cells from human T-lymphoma, dependent on IL-2, and human peripheral blood lymphocytes. The amount of MTT needed was 0.5 mg per well and the peak maximum of MTT formazan was close to 565 nm. The blastogenesis of the lymphocytes was expressed as a Stimulation Index (SI). The value of the SI was almost constant at cell numbers ranging from 5 x 10(5) to 1 x 10(6) cells/ml. The SI of some drugs using normal peripheral blood lymphocytes was less than 1.3. We used the MTT assay in the LST to examine the allergenicity of 83 drugs which were used in 43 cases of suspected drug-induced hepatitis. The range of SI was 0.92-2.02. An SI of 1.2 or more was seen in 62.8% of cases, 1.3 or more in 51.2% of cases, and 1.4 or more in 41.9% of cases. There were 26 drugs with SI greater than 1.3. Seven of these were antibiotics, while the rest included antihypertensives, analgesics, psychotropics, antiallergics and antiepileptics. This method is extremely well suited for the diagnosis of drug allergy in a clinical situation. The greatest benefit of the MTT assay is that the complete test procedures can be carried out in a general laboratory.