Long-term therapeutic effect of novel calcium phosphate-based compounds injected in ovariectomized rats.
Long-term therapeutic effect of novel calcium phosphate-based compounds injected in ovariectomized rats.
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DOI：
10.1002/jbm.b.31277
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Y. Tokudome;M. Otsuka;A. Ito;R. Legeros
中科院分区：
文献类型：
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作者：
Y. Tokudome;M. Otsuka;A. Ito;R. Legeros
本研究的具体目的是评价充分表征的Mg/Zn/F-CaP制剂（通过注射给药）在预防卵巢切除（OVX）大鼠骨矿物质缺乏症中的功效。将Donryu大鼠（4周龄，平均体重70 g）分为6个实验组：GN（正常），GC（对照，OVX）和OVX大鼠注射MZF-CaP（G2，G3和G4）或含锌磷酸三钙（ZnTCP，G1）的悬浮液。制备物的组成为G1：34.1重量% Ca; 19.5重量% P;和6.17重量% Zn; G2（#51）：23.7重量% Ca; 13.6重量% P; 1.1重量% Mg; 2.9重量% Zn，和1.1重量% F; G3（#68）：22.4重量% Ca; 14.1重量% P; 2.6重量% Mg、2.6重量% Zn和2.3重量% F; G4（#76）：28.5重量% Ca; 15.5重量% P; 1.9重量% Mg、1.8重量% Zn和3.0重量% F。每周一次将混悬液（10 mg/0.2 mL）注射至右大腿，持续12周。采用X线计算机断层扫描测量骨密度（BMD），采用股骨三点弯曲强度测量骨机械强度（BMS）。G1、G2、G3和G4组股骨的BMD和BMS显著高于GC组。任何组的左、右股骨BMD或BMS均无显著差异。结果表明，注射的Mg/Zn/F-CaP化合物有效地防止由卵巢切除术诱导的大鼠骨丢失，并表明这些化合物具有治疗骨质疏松症的潜在用途。
The specific aim of this study was to evaluate the efficacy of well-characterized Mg/Zn/F-CaP preparations (administered by injection) in preventing bone mineral deficiency in ovariectomized (OVX) rats. Donryu rats (4 weeks old, average weight 70 g) were divided into six experimental groups: GN (normal), GC (control, OVX), and OVX rats injected with suspensions of MZF-CaPs (G2, G3, and G4) or with Zn-containing tricalcium phosphate (ZnTCP, G1). The composition of the preparations was G1: 34.1 wt % Ca; 19.5 wt % P; and 6.17 wt % Zn; G2 (#51): 23.7 wt % Ca; 13.6 wt % P; 1.1 wt % Mg; 2.9 wt % Zn, and 1.1 wt % F; G3 (#68): 22.4 wt % Ca; 14.1 wt % P; 2.6 wt % Mg, 2.6 wt % Zn, and 2.3 wt % F; G4 (#76): 28.5 wt % Ca; 15.5 wt % P; 1.9 wt % Mg, 1.8 wt % Zn, and 3.0 wt % F. The suspensions (10 mg/0.2 mL) were injected into the right thigh once a week for 12 weeks. Bone mineral density (BMD) was measured by X-ray computed tomography and bone mechanical strength (BMS) was measured as femoral three-point bending strength. BMD and BMS were significantly higher in the femurs from groups G1, G2, G3, and G4 than from GC. No significant difference was observed in BMD or BMS between the left and right femurs for any group. The results indicate that the injected Mg/Zn/F-CaP compounds were effective in preventing bone loss induced by ovariectomy in rats and suggest that these compounds have potential use for treating osteoporosis.