Synthesis of poly(ethylene glycol)-silk fibroin conjugates and surface interaction between L-929 cells and the conjugates
Synthesis of poly(ethylene glycol)-silk fibroin conjugates and surface interaction between L-929 cells and the conjugates
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DOI：
10.1016/s0142-9612(96)00137-8
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发表时间：
1997-02-01
期刊：
影响因子：
14
通讯作者：
Imai, Y
中科院分区：
文献类型：
--
作者：
Gotoh, Y;Tsukada, M;Imai, Y
用2，4-二[O-甲氧基聚乙二醇]-6-氯-均三嗪（actPEG 2）在37 ℃下对溶解的丝素蛋白（SF）进行化学修饰，制备了聚乙二醇（PEG）-丝素蛋白（SF）缀合物（PEG 2-SF）。红外光谱和差示扫描量热（DSC）分析表明，PEG 2-SF和PEG 2-SF均通过改性引入到SF中，并通过甲醇水溶液处理诱导了SF和PEG 2-SF的β-折叠结构。根据在PEG 2-SF的DSC热分析图上观察到的PEG的熔融焓变化，评价PEG 2-SF中PEG组分的含量为67重量%。改性前后SF的含水量和接触角的测定表明，PEG 2-SF表面的亲水性比SF增加。采用细胞培养法研究了成纤维细胞（L-929）在PEG 2-SF基质上的贴壁和生长情况。PEG 2-SF表现出非常低的细胞附着和生长，尽管SF表现出高的细胞附着和生长。扫描电镜观察细胞聚集成团，未见丝状伪足。这些结果可以用PEG 2-SF表面的亲水性增加来解释。(C)1997年爱思唯尔科学有限公司。
Poly(ethylene glycol) (PEG)-silk fibroin (SF) conjugates (PEG2-SF) were prepared by the chemical modification of solubilized SF with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine (actPEG2) in berate buffer at 37 degrees C. The IR spectra and DSC curves of PEG2-SF and SF suggested the introduction of PEG into SF by the modification and the beta-sheet structure of both SF and PEG2-SF induced by the treatment with methanol aqueous solutions. The content of the PEG component in PEG2-SF was evaluated to be 67% by weight from the melting enthalpy change of PEG observed on the DSC thermogram of PEG2-SF. Water content and contact angle measurements of SF before and after the modification indicated that the hydrophilicity of the PEG2-SF surface increased compared with that of SF. The attachment and growth of fibroblast cells (L-929) on the matrix of PEG2-SF were studied by a cell culture method. PEG2-SF exhibited very low cell attachment and growth, though SF exhibited high cell attachment and growth. The filopodium of the cells attached to PEG2-SF could not be found, and the cells aggregated to form masses in scanning electron microscopy images. These results could be explained in terms of the increased hydrophilicity of the PEG2-SF surface. (C) 1997 Elsevier Science Limited.