Imaging plant tissues with coherent Raman scattering microscopy
Imaging plant tissues with coherent Raman scattering microscopy
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使用相干拉曼散射显微镜对植物组织进行成像
DOI：
10.1117/12.2001696
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Moger J
中科院分区：
文献类型：
--
作者：
Moger J
活植物组织的显微化学映射，而不使用外部标签将代表一个重大的进步，在分析能力的许多领域的生物研究;相干拉曼散射（CRS）显微镜提供了无标记的化学成像的基础上振动光谱，是一个显而易见的解决方案。然而，由于植物组织中的高水平的光吸收和荧光发射，该技术严重限制了体内植物成像。本文报告了与CRS在植物组织中进行无标记成像相关的技术问题的初步结果，并讨论了如何在未来的应用中减轻这些问题。
Microscopic chemical mapping of living plant tissues without the use of extrinsic labels would represent a major advance in analytical capability for many areas of biological research; Coherent Raman Scattering (CRS) microscopy offers label-free chemical imaging based on vibrational spectroscopy and is an obvious solution. However, due to the high levels of optical absorption and fluorescent emission in plant tissues the technique is severely limited for in-vivo plant imaging. This paper reports preliminary results regarding the technical issues associated with performing label-free imaging in plant tissues with CRS and discusses how they may be mitigated in future applications.