Walking Forward with Kinesin.
Walking Forward with Kinesin.
复制标题
与驱动蛋白一起前进。
DOI：
10.1016/j.tins.2018.07.006
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发表时间：
2018
影响因子：
15.9
通讯作者：
Holzbaur,ErikaLF
中科院分区：
文献类型：
--
作者：
Klinman,Eva;Holzbaur,ErikaLF
细胞器的主动细胞内运输依赖于细胞质动力蛋白和驱动蛋白，ATP依赖性微管马达蛋白的协调活动。虽然轴丝动力蛋白在20世纪60年代中期被发现，但直到20世纪80年代中期，驱动蛋白才被罗恩维尔和同事发现，如1985年报道的那样。他们的研究表明，从鱿鱼轴浆和牛脑中分离出的新鉴定的蛋白质能够独立地驱动微管滑动或细胞器运动。这些发现开启了生理学和神经科学领域的快速进展，导致了扩展驱动蛋白超家族的许多成员的鉴定，以及对其生物物理特性，细胞作用机制和疾病作用的详细探索。
Active intracellular transport of organelles relies on the coordinated activities of cytoplasmic dynein and kinesin, ATP-dependent microtubule motor proteins. While axonemal dynein was discovered during the mid-1960s, it was not until the mid-1980s that kinesin was discovered by Ron Vale and colleagues, as reported in 1985. Their research demonstrated that the newly identified protein, isolated from both squid axoplasm and bovine brain, was independently capable of driving microtubule gliding or organelle movement. These findings kicked off rapid progress in the fields of physiology and neuroscience, leading to the identification of the many members of the extended kinesin superfamily, as well as detailed explorations of their biophysical properties, cellular mechanisms of action, and roles in disease.