Rapid start-up of a nitrifying reactor using aerobic granular sludge as seed sludge.

Rapid start-up of a nitrifying reactor using aerobic granular sludge as seed sludge.
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DOI:
10.2166/wst.2012.888
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发表时间:
2012
期刊:
Water science and technology : a journal of the International Association on Water Pollution Research
影响因子:
--
通讯作者:
N. Kishida;Goro Saeki;S. Tsuneda;R. Sudo
N. Kishida;Goro Saeki;S. Tsuneda;R. Sudo
中科院分区:
其他
文献类型:
--
作者:
N. Kishida;Goro Saeki;S. Tsuneda;R. Sudo

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在实验室规模的连续搅拌反应器(CSTR)中,采用全无机高浓度氨氮废水,研究了好氧颗粒污泥作为种子污泥快速启动硝化过程的效果。即使在反应器内接种大量颗粒生物质,进水水质发生突变,也没有出现过多的生物质冲刷现象,由于好氧颗粒污泥具有较高的沉降能力,因此在启动阶段仍能保持较高的生物质浓度。结果,氨氮的去除速率立即提高,在20天内达到1.0 kg N/m(3)/d以上,第39天达到1.8 kg N/m(3)/d。随后,在100d内稳定地实现了高速亚硝化。然而,在达到完全硝化为硝酸盐之前,观察到亚硝酸盐积累了140天。荧光原位杂交分析表明,在启动过程中,颗粒污泥外缘存在的氨氧化细菌数量增加。这种微生物生态变化将使实现高效率的氨氮去除成为可能。
In this study, the effectiveness of aerobic granular sludge as seed sludge for rapid start-up of nitrifying processes was investigated using a laboratory-scale continuous stirred-tank reactor (CSTR) fed with completely inorganic wastewater which contained a high concentration of ammonia. Even when a large amount of granular biomass was inoculated in the reactor, and the characteristics of influent wastewater were abruptly changed, excess biomass washout was not observed, and biomass concentration was kept high at the start-up period due to high settling ability of the aerobic granular sludge. As a result, an ammonia removal rate immediately increased and reached more than 1.0 kg N/m(3)/d within 20 days and up to 1.8 kg N/m(3)/d on day 39. Subsequently, high rate nitritation was stably attained during 100 days. However, nitrite accumulation had been observed for 140 days before attaining complete nitrification to nitrate. Fluorescence in situ hybridization analysis revealed the increase in amount of ammonia-oxidizing bacteria which existed in the outer edge of the granular sludge during the start-up period. This microbial ecological change would make it possible to attain high rate ammonia removal.