Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis
Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis
复制标题
CIK受体激酶在调节拟南芥干细胞信号传导中的保守和分化功能
DOI：
10.1016/j.molp.2021.04.001
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发表时间：
2021
期刊：
影响因子：
27.5
通讯作者：
Gou Xiaoping
中科院分区：
文献类型：
--
作者：
Zhu Yafen;Hu Chong;Cui Yanwei;Zeng Li;Li Sunjingnan;Zhu Mingsong;Meng Fanhui;Huang Shuting;Long Li;Yi Jing;Li Jia;Gou Xiaoping
在高等植物中，顶端分生组织(SAM)和根尖分生组织(RAM)分别是形成地上和地下组织和器官的干细胞池。CLAVATA3(CLV3)-WUSCHEL(WUS)负反馈环是控制SAM动态平衡的核心途径，而CLV3/胚胎周围区(ESR)40(CLE40)和WUSCHEL相关的HOMEOBOX5(WOX5)是CLV3和WUS的同源物，直接影响小柱干细胞的命运。此外，CLV3不敏感蛋白激酶(CIK)被证明是维持SAM动态平衡所必需的，但它们是否调节远端根分生组织仍有待阐明。在这里，我们报告了CIK对于传递CLE40信号以维持远端根分生组织的动态平衡是必不可少的。我们发现，CIK突变体的根表现出静止中心的紊乱和柱状干细胞(CSC)分化的延迟。生化分析表明，CIKs与拟南芥CRINKLY4(ACR4)以非配体依赖的方式相互作用，并能被ACR4体外磷酸化。此外，部分依赖于ACR4的CLE40能迅速诱导CIKs的磷酸化。尽管CIK在SAM和RAM中起着保守和冗余的调节作用，但我们的结果表明它们在这些分生组织中表现出不同的功能。
The shoot apicalmeristem (SAM) and root apical meristem (RAM) act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants, respectively. The CLAVATA3 (CLV3)- WUSCHEL (WUS) negative-feedback loop acts as a core pathway controlling SAM homeostasis, while CLV3/EMBRYO SURROUNDING REGION (ESR) 40 (CLE40) and WUSCHEL-RELATED HOMEOBOX5 (WOX5), homologs of CLV3 and WUS, direct columella stem cell fate. Moreover, CLV3 INSENSITIVE KINASES (CIKs) have been shown to be essential for maintaining SAM homeostasis, whereas whether they regulate the distal root meristem remains to be elucidated. Here, we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem. We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell (CSC) differentiation. Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4 (ACR4) in a ligand-independent manner and can be phosphorylated by ACR4 in vitro. In addition, the phosphorylation of CIKs can be rapidly induced by CLE40, which partially depends on ACR4. Although CIKs act as conserved and redundant regulators in the SAM and RAM, our results demonstrated that they exhibit differentiated functions in these meristems.