Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections.
Notes on the application of microwaves for antigen retrieval in paraffin and plastic tissue sections.
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关于石蜡和塑料组织切片中使用微波进行抗原修复的注意事项。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
M. Boon
中科院分区：
文献类型：
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作者：
A. Suurmeijer;M. Boon
在福尔马林固定，石蜡包埋组织增强或从头免疫染色可以获得微波煮沸的切片在金属盐或缓冲溶液。本文对这一新技术进行了综述，并对影响最终结果的重要因素进行了讨论。微波抗原提取也可以应用于塑料GMA切片的免疫组织化学。在这里，微波法的作用可能主要是由于破坏了GMA和禁止免疫染色的蛋白质之间的键。微波法不需要胰蛋白酶处理。
In formalin-fixed, paraffin-embedded tissue enhanced or de novo immunostaining can be obtained by microwave boiling of sections in a metal salt or buffer solution. In this paper this new technique is reviewed and important factors influencing final results are discussed. Microwave antigen retrieval can also be applied for immunohistochemistry on plastic GMA sections. Here the action of the microwave method is probably mainly due to breaking the bonds between GMA and proteins prohibiting immunostaining. The microwave methods do not require trypsin treatment.